Sequences from confirmation amplicon


Confirmation sequences for line 399F01 and hit At4g03390 are shown.
Line ID 399F01
Locus At4g03390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1491119
Primers used for PCR EQ49 - 8474, show primer details
   
Sequence Name 11-K103074-0022-399-F01C-EQ49
GenBank Accession LN491067 [GenBank]
Sequencing primer gene specific primer, EQ49: 5'-CAACACAGAGCAAGTTCTATGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103074-0022-399-F01C-EQ49
GTTCAAGAACGAGTAACACACCATTTTACAGTAGAGGTCTTCAACCAATACATATTACTA
ACACAGATGATTCAAGATTCACAAAAAGAAACTCTGCACCAATCGAATCATCGCCCATTG
GAAGCAAGCACATTGATCCTTTCCCCTGAGCCACTTCACAAGGCTGTAGAATCAGCTTAA
GCCTCCTTAAGCCTCCTTCTGAGGCTTTGGCTTTTTTTTTTTTTTTTAGGGGTCGGGTTT
ATTCCCCTATTTTTTTTTTTTTTTTTCAAAAAAAAAAAATTTTTTTGTTTCCCCAAATTC
CCAAAAATAACCCAAAAAAAAAAGGTTTTTTTAAAATTCATTATCCCGGGGAAAAACCCC
CCAAACCAGGACCCTTTTTTTGAATAAATTTTGGAAAATTTGAAAAAATTTCGAACATCG
GGGGTTAAAATTCGGGTTCAAACAAAAAAAAAAAAAAAATTTTTTTGGTCCCCCAAACCA
AATCACAAGCTTTTAACCCCGTTTTCAAATTGCCAGTAGGTTTTTTCCCGAAACCCCCCG
GGATTCCCAATTGAAAAGGGCTTCTTGCCCGGAAAATCTCCGGGGATCACCAAGGGTTAT
GAGGGCCAATTGGGAAAAAAAAAAAAAGTATTTCCCATTTTTTTTTCATTTCAAAAAGGT
AAATTTCCC
   
Sequence Name 12-K103074-0022-399-F01C-8409
GenBank Accession LN491531 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103074-0022-399-F01C-8409
TCGGACATGAGCCTTTACAATTGGATATCCCGGTGTGTTCAGGTAAAACACATACTGACA
ATTAGAAACTGTGGTTAGATGCTTGTGATTGTGTTTGCGTGACCAAAAGAGTTTTATTTC
TTTTCTAGTCTGAACCAGAATTTAGACCACTGATGTCAGAAGTTGTTCAAGATCTACTAG
ATATGATCAGAAGAGAGCGTCATGGTTCGGGTGATTCTACCGCGGACTAGTGAATTGTAA
AGAAGCATTTTTTTATCGGATCATCTCTGTGAATTCGTGGAAACAAGAGATGTTCAGTTC
TCAGAAACAACAACAACAACATAGGGAACTAAACCAGACACATAAAAAAAAAAAAAAAAC
CCAAACCCCCAAAAGGGGGTTTAGGGGGGCTTAACCTGATTCCCCCCCCTTGGAAAGGGG
CCCGGGGAAAAGGATAAAGGGGTTTGCTTCCAGGGGGGAAAAATTCAATGGGGGCAAATT
TTTTTTTTGGGAATCTGGAACCCCCGGGGTTAAAAAAATGTTTGGGTGAAAAACCCCCCC
GGAAAAAGGGGGGGTTCCCCGATTTTTTGAACTTGTTTTATAAAAACTTTAAAACCCCAA
AAAATTTGC
Primers used for PCR 06cj - 8474, show primer details
   
Sequence Name 78-K118629-0022-399-F01C-06cj
GenBank Accession LN513750 [GenBank]
Sequencing primer gene specific primer, 06cj: 5'-TCAAAGAATCGAGTAACACACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118629-0022-399-F01C-06cj
ATTACTAACCAGATGATTCAAGATTCACAAAAAGAAACTCTGCACCAATCGAATCATCGC
CCATTGGAAGCAAGCACATTGATCCTTTCCCCTGAGCCACTTCACAAGGCTGTAGAATCA
GCTTAAGCCTCCTTAAGCCTCCTTCTGAGGCTTTGGCTTTTTTTTTTTTTTTTAGGGGCC
GGGTTTATTCCCCTATGTTGTTTTTTTTTTTTCAAAAAACGAAACTTTTTTGGTTTCCCC
AAATTCCCAAAAATAACCCAAAAAAAAAAGGTTTTTTTACATTTCATTATCCCGGGGAAA
AATCCCCCAAACCAGGACCTTTTTTTCGAATAATTTTTGGAAAATTTGAAAAAATTTCGA
ACATCGGGGGTCAAATTTTGGGTTCAAACAAAAAAAAAAAAAAAATTTTTTGGGTCCCCC
AAACAAATTCCAAAGCTTTTAACCCCATTTTTTAATTGCCAGTAGGTTTTTTCCCGAAAC
CCCCGGGGATTCCCATTTGAAAAGGGTTTCTGGCCCGGAAAATCTCCGGGGATAACCATT
GATTTTAAGGGCCAATAGGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTCAA
AATTGAAAATTTCCCCACCGTTTTTAAAAA
   
Sequence Name 59-K118628-0022-399-F01C-8409
GenBank Accession LN510110 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118628-0022-399-F01C-8409
TTTCAATTGGATATCCCGGTGTGTTCAGGTAAAACACATACTGACAATTAGAAACTGTGG
TTAGATGCTTGTGATTGTGTTTGCGTGACCAAAAGAGTTTTATTTCTTTTCTAGTCTGAA
CCAGAATTTAGACCACTGATGTCAGAAGTTGTTCAAGATCTACTAGATATGATCAGAAGA
GAGCGTCATGGTTCGGGTGATTCTACCGCGGACTAGTGAATTGTAAAGAAGCATTTTTTT
ATCGGATCATCTCTGTGAATTCGTGGAAACAAGAGATGTTCAGTTCTCAGAAACAACAAC
AACAACATAGGGAACTAAACCAGACACATAAAAAAAAAAAAAAAACCCAAACCCCCAAAG
GGGGGCTTAGGGGGGCTTAACCTGATTCTCCCCCCTTGGAAAGGGGCCCGGGGAAAAGGA
TCATGGGGCTTGCTTCCAGGGGGGAAAAATCCAATGGGGGCAAATTTTTTTTTGGGGAAT
CTGGAACCCCCGGGGTAAAAAAAATGTTTGGGTGAAAAACCCCCCCGGAAAAAGGGGGGG
TTACC
Primers used for PCR 40wq - 8474, show primer details
   
Sequence Name 79-K118629-0022-399-F01C-40wq
GenBank Accession LN513880 [GenBank]
Sequencing primer gene specific primer, 40wq: 5'-GAATTCAAGAAGAAACTTTCGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K118629-0022-399-F01C-40wq
GTTCTTTTATTTACTTCAAATGGAAGTGTCCAACTGTGTTTTCTACCTGATTGCTAACTT
TTGTTAATGCTTTCAAGGTACTTGCATGAGGTGTGTGAACCACCTATCATACACAGAAAC
TTCAAATCAGCCAATGTTCTACTCGACGATGACCTGAGTGTTCTTGTCTCAGATTGTGGT
TTGGCCCCACTAATATCATCAGGCTCTGTGAGTCAGGTAAAATACTTAAACCCAATGAGC
TTGATTCTGATGGATATACCCTCTACAGATTTCAAGATGTTAGACTAAACTATTTCTTAT
AATGATAATCAGTTATCAGGGCAATTGCTAGCCGCTTACGGATATGGAGCTCCAGAGTTC
GACTCTGGAATCTATACATGGCAGAGTGATGTATATAGTTTTGGTGTTGTTATGTTAGAA
CTATTGACGGGTAGAATGTCCTACGATAGGTATGCATGTCACATTGTTTTATTTGCTTAG
TTTTCTGTTTCCGGTTAAGCATTTATTCATATCAACGAGTCGATTATAGGGACCGGAGTA
GAGGAGAGCAGTTCTTGGTGAGGTGGGCAATCCCACAGCTTCATGATATCGATGCATTGG
GCAAAATGGTTGATCCATCTCTTAATGGACAATACCCTGCAAAGTCATTGTCACACTTTG
CTGATATCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGC
   
Sequence Name 60-K118628-0022-399-F01C-oriL
GenBank Accession LN510376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118628-0022-399-F01C-oriL
CATTTACAATTGGATATCAGCAAAGTGTGACAATGACTTTGCAGGGTATTGTCCATTAAG
AGATGGATCAACCATTTTGCCCAATGCATCGATATCATGAAGCTGTGGGATTGCCCACCT
CACCAAGAACTGCTCTCCTCTACTCCGGTCCCTATAATCGACTCGTTGATATGAATAAAT
GCTTAACCGGAAACAGAAAACTAAGCAAATAAAACAATGTGACATGCATACCTATCGTAG
GACATTCTACCCGTCAATAGTTCTAACATAACAACACCAAAACTATATACATCACTCTGC
CATGTATAGATTCCAGAGTCGAACTCTGGAGCTCCATATCCGTAAGCGGCTAGCAATTGC
CCTGATAACTGATTATCATTATAAGAAATAGTTTAGTCTAACATCTTGAAATCTGTAGAG
GGTATATCCATCAGAATCAAGCTCATTGGGTTTAAGTATTTTACCTGACTCACAGAGCCT
GATGATATTAGTGGGGCCAAACCACAATCTGAGACAAGAACACTCAGGTCATCGTCGAGT
AGAACATTGGCTGATTTGAAGTTTCTGTGTATGATAGGTGGTTCACACACCTCATGCAAG
TACCTTGAAAGCATTAACAAAAGTTAGCAATCAGGTAGAAAACACAGTTGGACACTTCCA
TTTTGAAGTAAATAAAAGAACTTACTCAAGGGCTCTAGCAGCTCCAAGTGCCATACTGAC
ACGGGTATTCCACGAAAGTTTCTTCCTTGAATTC

Last Updated on 06.12.2018 16:11