Sequences from confirmation amplicon


Confirmation sequences for line 399F09 and hit At4g36480 are shown.
Line ID 399F09
Locus At4g36480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17220786
Primers used for PCR HV66 - 8474, show primer details
   
Sequence Name 52-K101270-0022-399-F09C-OriL
GenBank Accession LN507755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101270-0022-399-F09C-OriL
ATTTACATTGAAATATCCATATTGTTTTCTTAAAATTAGAGAAATCTAGCGGTTCAGCTA
AAGATGATTTGCTTCTACTTGAGAAAATGGCTGACCGTGTAAGTTCATTGTTTCTTTGCA
AAACCAAATCTAGACCCTGTCCCCGTTTCTTGACATGACTAGATGCATGCAGGCTCTGAA
GGAAGACTCATTGTTGGTTGTGAGTTCTAAAAGATCATTTCTTGATAAATGCCGGTTACC
TGTGGGAATCAAACTCTACGTCTCAGCGGGACATTCGGAATCCGATCTTCTTAAAGCGTC
GGAGTCACTGAAGAGACTTGCTTCAGAGCTTCTACTCAAGTCCTGAAACACCGAAACTCT
TTTTTCTTCAAGAACAACAACACTGAAAGGAACTC
   
Sequence Name 52-K101276-0022-399-F09C-HV66
GenBank Accession LN507756 [GenBank]
Sequencing primer gene specific primer, HV66: 5'-AAGAGTTCCCTTCAGTGTTGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101276-0022-399-F09C-HV66
ACTTGAGTGAAGCTCTGAAGCAAGTCTCTTCAGTGACTCCGACGCTTTAAGAAGATCGGA
TTCCGAATGTCCCGCTGAGACGTAGAGTTTGATTCCCACAGGTAACCGGCATTTATCAAG
AAATGATCTTTTAGAACTCACAACCAACAATGAGTCTTCCTTCAGAGCCTGCATGCATCT
AGTCATGTCAAGAAACGGGGACAGGGTCTAGATTTGGTTTTGCAAAGAAACAATGAACTT
ACACGGTCAGCCATTTTCTCAAGTAGAAGCAAATCATCTTTAGCTGAACCGCTNGATTTC
TCTAATTTTAAGAAAACAATATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
Primers used for PCR HV65 - 8474, show primer details
   
Sequence Name 51-K101270-0022-399-F09C-8409
GenBank Accession LN507441 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101270-0022-399-F09C-8409
TTCGGACTGAGCCATTTACATTGAATATATCCTGCGTTGCTTGTTAATGACATCCCCTTG
ATATCTGACAATCCTGCGTTGTTCCCAGCATTTCTTTTTCAATTAGAGGAAGATAACAAA
CTAAATCAAACTCTAATTGTTGCTAGATTAGATTAAAACTTGAAGATCAAACCTTTCCAT
AACAGAGCAACATTTTGCTTCAGCTTAACTAACATATCAGGGTTTTGATCAATGACATCA
ATGGCGGTGATAGCAGCACTTGCAAGGTATGGTGGTAAAGAAGCAGAGAACACATATCCT
GAACTGCTAAGTCTCTGTGCATTTGAGCGTCCCAGAGAAAAGTTTCTATTATCGGTAGTT
TTTTAATTTTGTTTGTAATTAGTTAATCAGCATGATCGATGTTTACCTGGTAATCGATGA
TGCGGGCATTCCCAGTGCAAAATCCACCTTCTGTGGCTAATGCATGGCCCATTGCAGCAG
TCACAACATCTATCTTCTCAATCTGTTTAAAGATAAACCGCCGTGAAATTTTTAGCATCA
GGTTTGGCAAATGAGAACACTACTAANATGTGTGTCAATCATACTTACAGGGACACTATG
ATGCTCTG

Last Updated on 06.12.2018 16:11