Sequences from confirmation amplicon


Confirmation sequences for line 401E01 and hit At5g60740 are shown.
Line ID 401E01
Locus At5g60740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24426338
Primers used for PCR MD12 - 8474, show primer details
   
Sequence Name 21-K024845-022-401-E01C-8409
GenBank Accession LN495505 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K024845-022-401-E01C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTAGTATGGCCAGGTTCGAAA
AGTATGGCCAGGGTATAAGCTATACCAGTCACACAGTATACAAGACAGATGAGGACAACA
TAATTGTCTGTAACTGTTGATCTTGGATTGTTGAAGAAGTAGAACATGGATAGATAAACA
AGCGGCTTAACGATGGTGTTGAAGTGATCAACTGTGTCTTTCGCTAGGAAGTAAGCCAGG
CTGCTCATTCCGGCTCGGCTTTCTCTCCAATAGTGTAGTTTATCCAGAGAGAAAGATCTT
AATGCAGTAATCTTACACAAGAGAGCTGAAAAAAACAAAAAATTGTTGCCAGAATAGATG
AAAGAAGCAAAGTTGTTGTCAAAATAGCTTGAAAGTTTCTACTTACAAACAGCAATAACT
GTATATGTATATCCCATGGCACCAAACGTTTCATCGCTCACTTTAGCAAGCGTTCCTAAG
CAGATACCAGCAAGTAAAAGTATTAGGTAATCTACAGCTAGTGTCCTTGCTTCTCGTAGT
CTTTGTTTACCTAACCTGCAAAGCAAACACATCGAGGTTAAGAAAAGGATTCTCTGTGAA
TCGATTGATTTTGCAAGTATTTGGAAGGGATCTACCTGTCCTAAGAAGTATCTGTATTGC
TGATACACACCAGGAACTTCTCTTTCGGA
   
Sequence Name 22-K024845-022-401-E01C-MD12
GenBank Accession LN495952 [GenBank]
Sequencing primer gene specific primer, MD12: 5'-AGTTCAGGTGACTTATCCGAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K024845-022-401-E01C-MD12
TGGTGTGTATCAGCAATACAGATACTTCTTAGGAAGGTAGATCCCTTCCAAATACTTGCA
AAATCAATCGATTCACAGAGAATCCTTTTCTTAACCTCGATGTGTTTGCTTTGCAGGTTA
GGTAAACAAAGACTACGAGAAGCAAGGACACTAGCTGTAGATTACCTAATACTTTTACTT
GCTGGTATCTGCTTAGGAACGCTTGCTAAAGTGAGCGATGAAACGTTTGGTGCCATGGGA
TATACATATACAGTTATTGCTGTTTGTAAGTAGAAACTTTCAAGCTATTTTGACAACAAC
TTTGCTTCTTTCATCTATTCTGGCAACAATTTTTTGTTTTTTTCAGCTCTCTTGTGTAAG
ATTACTGCATTAAGATCTTTCTCTCTGGATAAACTACACTATTGGAGAGAAAGCCGAGCC
GGAATGAGCAGCCTGGCTTACTTCCTAGCGAAAGACACAGTTGATCACTTCAACACCATC
GTTAAGCCGCTTGTTTATCTATCCATGTTCTACTTCTTCAACAATCCAAGATCAACAGTT
ACAGACAATTATGTTGTCCTCATCTGTCTTGTATACTGTGTGACTGGTATAGCTTATACC
CTGGCCATACTTTTCGAACCTGGCCATACTAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAATGTAGATGT

Last Updated on 06.12.2018 16:11