Sequences from confirmation amplicon


Confirmation sequences for line 402B01 and hit At1g68650 are shown.
Line ID 402B01
Locus At1g68650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25775763
Primers used for PCR RK76 - 8474, show primer details
   
Sequence Name 25-K021337-022-402-B01C-8409
GenBank Accession LN497251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K021337-022-402-B01C-8409
ATTCACCTTCTCTTACGCGACTCTCGCCGGAAACAGATCGCTCCGCCGTGAACTCGGAAG
CCATGGGGTCACTTTTGCAGGTACGTATGATCCAGTTGGAAGTTTAATCTGTTCTCTGAA
AATTCGAAGTTTGTAACTTTTGGAACCGAAGCTAATTGATGGGTTTCTTTAGGGTTTTAC
TAAATCACTCGCCATGACTTTCCTCTCCGAGATTGGTGACAAAACGTTCTTCGCTGCTGC
TGTAAGTGAATCTTAATCATCTTCTTCCTCTTCATATTTGGAGTTAAAAAGAGTATACTT
TTAATGGCGAGATCAATATTCTAAAATCCTCAAAGCTTAAAGCTTTAGCAACATTCATAA
TTCAATATGAACGTTTCTTTGTTAATTTTGGAGCCAATGTTTCTAGTTAATGCTGAAATG
GTTATGTTCTTTGATCCAACTCTGTTGACTTTTTGTTACTAAAGCTTCTTATTGTTGAGC
TAACTATTTATCTCCTCAGCTTCGGTATATG
   
Sequence Name 26-K021337-022-402-B01C-RK76
GenBank Accession LN497728 [GenBank]
Sequencing primer gene specific primer, RK76: 5'-AAAACATATACCGAAGCTGAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K021337-022-402-B01C-RK76
CATAGTTAGCTCAACAATAAGAAGCTTTAGTAACAAAAAGTCAACAGAGTTGGATCAAAG
AACATAACCATTTCAGCATTAACTAGAAACATTGGCTCCAAAATTAACAAAGAAACGTTC
ATATTGAATTATGAATGTTGCTAAAGCTTTAAGCTTTGAGGATTTTAGAATATTGATCTC
GCCATTAAAAGTATACTCTTTTTAACTCCAAATATGAAGAGGAAGAAGATGATTAAGATT
CACTTACAGCAGCAGCGAAGAACGTTTTGTCACCAATCTCGGAGAGGAAAGTCATGGCGA
GTGATTTAGTAAAACCCTAAAGAAACCCATCAATTAGCTTCGGTTCCAAAAGTTACAAAC
TTCGAATTTTCAGAGAACAGATTAAACTTCCAACTGGATCATACGTACCTGCAAAAGTGA
CCCCATGGCTTCCGAGTTCACGGCGGAGCGATCTGTTTCCGGCGAGAGTCGCGTAAGAGA
AGGTGAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11