Sequences from confirmation amplicon


Confirmation sequences for line 402G03 and hit At2g21990 are shown.
Line ID 402G03
Locus At2g21990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9360085
Primers used for PCR JG82 - 8474, show primer details
   
Sequence Name 50-K034069-022-402-G03C-8409
GenBank Accession LN507083 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K034069-022-402-G03C-8409
CCCGGACAGCACCGAAGCGGGTCAAGCGGATCAAGAGTTACCGGAACCTTATTCGGATAC
CGTAAAGGCCGTGTAAGCCTCTCGATTCAAGAAAGCCCTAGATGTTTACCATCTCTCGTC
GTCGAGCTCGCAATGCAAACAATGGTGCTACAAAAAGAGCTTAGCGGAGGGATGGTTAGG
ATCGCGTTAGAGACAGAGAAACGAGGTGATAAAGAGAAGATCAAGATCATGGACGAGCCA
TTATGGACAATGTTTAGTAACGGTAAGAAAACGGGGTACGGTGTGAAACGTGACGCGACG
GAGGAAGACCTCAACGTTATGGAGCTATTAAGACCGGTTTCGATGGGTGCCGGAGTTTTG
CCAGGGAACACGGAGTTTGAAGGACCAGACTCGGAGATGGCTTACATGAGGGCTTACTTC
GAACGAGTCGTTGGGTCTAGAAATTCAG
   
Sequence Name 66-K034069-022-402-G03C-JG82
GenBank Accession LN511538 [GenBank]
Sequencing primer gene specific primer, JG82: 5'-CTCTGAATCTTTAGACCCAACGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K034069-022-402-G03C-JG82
GTAAAGCCATCTCCGAGTCTGGTCCTTCAAACTCCGTGTTCCCTGGCAAAACTCCGGCAC
CCATCGAAACCGGTCTTAATAGCTCCATAACGTTGAGGTCTTCCTCCGTCGCGTCACGTT
TCACACCGTACCCCGTTTTCTTACCGTTACTAAACATTGTCCATAATGGCTCGTCCATGA
TCTTGATCTTCTCTTTATCACCTCGTTTCTCTGTCTCTAACGCGATCCTAACCATCCCTC
CGCTAAGCTCTTTTTGTAGCACCATTGTTTGCATTGCGAGCTCGACGACGAGAGATGGTA
AACATCTAGGGCTTTCTTGAATCGAGAGGCTTACACGGCCTTTACGGTATCCGAATAAGG
TTCCGGTAACTCTTGATCCGCTTGACCCGCTTCGGTGCTGGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11