Sequences from confirmation amplicon


Confirmation sequences for line 403B02 and hit At2g25880 are shown.
Line ID 403B02
Locus At2g25880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11036246
Primers used for PCR DF02 - 8474, show primer details
   
Sequence Name 29-K029490-022-403-B02C-8409
GenBank Accession LN499159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K029490-022-403-B02C-8409
TTTACATTTTTCTCCATTAGTGTTTCTTCACAATTATGTTAAACAAGCAGAATTAATTTG
ATGTCTTTTTTAACCAAGCTGTCTTCTATGTGTCTTTGCAAACAGAGCGATCACATTGTG
GCTTTAAAGGTTCTTTTTAAGGCTCAGCTTCAACAATCTCAAGTTGAACACCAGTTACGA
AGGGAAGTAGAGATTCAATCTCATCTTCGGCATCCCAATATTCTGCGGCTCTACGGTTAC
TTCTATGATCAGGTTTTGTGTTATCTGTGCTTGTAAAAGAACTTCTTGTTGACATACAAT
GTTTGGCTCTGTGAGGTTAACATAGTTTTTGGGATACCGTTGTTGCAGAAAAGAGTTTAT
TTGATTTTGGAGTATGCTGTAAGGGGTGAGCTTTATAAAGAGCTTCAGAAGTGCAAGTAC
TTCAGCGAACGTCGAGCAGCAACTGTAAGTCCTAGTGATAATTGAACTTATTCTAGCATG
TTAATGTGAATCTGTGTAGTCATACCCTTACGTTTAT
   
Sequence Name 30-K029490-022-403-B02C-DF02
GenBank Accession LN499594 [GenBank]
Sequencing primer gene specific primer, DF02: 5'-ATACTGCCACAATAAACGTAAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K029490-022-403-B02C-DF02
CATTACATGCTAGAAAAGTTCAATTATCACTAGGACTTACAGTTGCTGCTCGACGTTCGC
TGAAGTACTTGCACTTCTGAAGCTCTTTATAAAGCTCACCCCTTACAGCATACTCCAAAA
TCAAATAAACTCTTTTCTGCAACAACGGTATCCCAAAAACTATGTTAACCTCACAGAGCC
AAACATTGTATGTCAACAAGAAGTTCTTTTACAAGCACAGATAACACAAAACCTGATCAT
AGAAGTAACCGTAGAGCCGCAGAATATTGGGATGCCGAAGATGAGATTGAATCTCTACTT
CCCTTCGTAACTGGTGTTCAACTTGAGATTGTTGAAGCTGAGCCTTAAAAAGAACCTTTA
AAGCCACAATGTGATCGCTCTGTTTGCAAAGACACATAGAAGACAGCTTGGTTAAAAAAG
ACATCAAATTAATTCTGCTTGTTTAACATAATTGTGAAGAAACACTAATGGAGAAAAATG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR MJ66 - 8474, show primer details
   
Sequence Name 08-K029636-022-403-B02C-8409
GenBank Accession LN489605 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K029636-022-403-B02C-8409
GCCATTTACATTTTTCTCCATTAGTGTTTCTTCACAATTATGTTAAACAAGCAGAATTAA
TTTGATGTCTTTTTAACCAAGCTGTCTTCTATGTGTCTTTGCAAACAGAGCGATCACATT
GTGGCTTTAAAGGTTCTTTTTAAGGCTCAGCTTCAACAATCTCAAGTTGAACACCAGTTA
CGAAGGGAAGTAGAGATTCAATCTCATCTTCGGCATCCCAATATTCTGCGGCTCTACGGT
TACTTCTATGATCAGGTTTTGTGTTATCTGTGCTTGTAAAAGAACTTCTTGTTGACATAC
AATGTTTGGCTCTGTGAGGTTAACATAGTTTTTGGGATACCGTTGTTGCAGAAAAGAGTT
TATTTGATTTTGGAGTATGCTGTAAGGGGTGACTTTAATAA
   
Sequence Name 09-K029636-022-403-B02C-MJ66
GenBank Accession LN490048 [GenBank]
Sequencing primer gene specific primer, MJ66: 5'-TTTATAAAGCTCACCCCTTACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K029636-022-403-B02C-MJ66
CTTTTCTGCAACAACGGTTATCCCAAAAACTATGTTAACCTCACAAAGCCAAACATTGTA
TGTCAACAAGAAGTTCTTTTACAAGCACAGATAACACAAAACCTGATCATAGAAGTAACC
GTAAAGCCGCAAAATATTGGGATGCCAAAAATGAGATTGAATCTCTACTTCCCTTCGTAA
CTGGGGTTCAACTTGAGATTGTTGAAGCTGAGCCTTAAAAAGAACCTTTAAAGCCCCAAG
GTGATCGCTCTGTTTGCAAAGACACATAGAAAACAGCTGGGTTAAAAAAGACATCAAATT
AATTCTGCTTGTTTAACATAATTGTGAAGAAACACTAATGGAGAAAAATGTAAATGGCTT
CTTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTCCCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCACCGTTTTTATAA
AATGAAAGTACATTTTGATAAAACAACAAATTACGATCCGTCGTATTTATAGGCAAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTT
Primers used for PCR FN63 - 8474, show primer details
   
Sequence Name 24-K035334-022-403-B02C-FN63
GenBank Accession LN496905 [GenBank]
Sequencing primer gene specific primer, FN63: 5'-AGGGTTTTACTTCCTTGTTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K035334-022-403-B02C-FN63
TTCTATGGAATGTGATCTGGGATTATCGATTTGGTTGCTTGATTTTCTTTGCGCCAGAAA
GAATCTTGTTTCAATGTTCTCTGTTTAATTTGAGAAAGACTTGTTGGTATGATTTTGAGA
AATTTCATGATATGATTGTGGATTTATTCGTTTACAGCGAGGACCCTAGATTAATTGACA
AATCCGTGGATTAGTGTTAAGGAGCTGTTGATTTTATCCTTTTATCTTCAAGTTTTAAAG
TATCAATGATTGTTTGTCGTTTATGATTAAGTGTATCTGAGAGTTGATTGGATTTCAATC
TTTGTGATAATGTTGTATCAGGCGGCTTCAGAGGCTGCACAAAAGAGATGGACTACAAGC
GATTTCGATATCGGAAAGCCACTTGGTAGAGGAAAGTTTGGCCATGTTTATCTAGCAAGA
GAGAAACGGGTTAGTGTTTCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 24-K035334-022-403-B02C-8409
GenBank Accession LN496904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K035334-022-403-B02C-8409
CGTTTCTCTCTTGCTAGATAACATGGCCAAACTTTCCTCTACCAAGTGGCTTTCCGATAT
CGAAATCGCTTGTAGTCCATCTCTTTTGTGCAGCCTCTGAAGCCGCCTGATACAACATTA
TCACAAAGATTGAAATCCAATCAACTCTCAGATACACTTAATCATAAACGACAAACAATC
ATTGATACTTTAAAACTTGAAGATAAAAGGATAAAATCAACAGCTCCTTAACACTAATCC
ACGGATTTGTCAATTAATCTAGGGTCCTCGCTGTAAACGAATAAATCCACAATCATATCA
TGAAATTTCTCAAAATCATACCAACAAGTCTTTCTCAAATTAAACAGAGAACATTGAAAC
AAGATTCTTTCTGGCGCAAAGAAAATCAAGCAACCAAATCGATAATCCCAGATCACATTC
CATTAGAACAGACCAAACGCAGAGAAAAAACGCAAACCAACAAGGAAGTAAAA

Last Updated on 06.12.2018 16:11