Sequences from confirmation amplicon


Confirmation sequences for line 405E03 and hit At2g24740 are shown.
Line ID 405E03
Locus At2g24740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10531876
Primers used for PCR IN37 - 8474, show primer details
   
Sequence Name 25-K033706-022-405-E03C-8409
GenBank Accession LN497341 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K033706-022-405-E03C-8409
AAATCTGATGCTGTTATGAAGAAGCCCACGCAACATCAGTACCTCCTCATGCACCGCCAC
AAACCCTTTGGCTATTAGATAACTTCAACATTGAGGACTCTTATGACCGAGATGCTGGTC
CTAGTACTGGTCCAGTCCACAGAGAAAGATCAGATGCTGTTAATGAAGAAGCCCACGCAA
CATCAATACCTCCTCATGCACCGCCACAAACCCTTTGGCTATTAGATAACTTCAACATTG
AGGACTCTTATGACCGAGATGCCGGTCCTAGTACTAGTCCAATAGACCGAGAAGCATCTC
ATGAAGTCAATGAAGACGCCCACGCAACATCAGCACCTCCTCATGTAATGGTCTCACCAC
TACAAAACAGAAGACCATTTGATCAATTCAACAACCAACCTTATGATGCGAGTGCTGGTC
CTAGTACTGGTCCAGGAAAGCGAGGACGTGGCCGACCAAAGGGTTCGAAAAATGGGTCGA
GAAAGCCAAAAAAGCCAAAAGCATATGACAACAACTCTACTGATGCGAGTGCTGGTCCTA
GTTCTGGTCTAGGAAAAGNAAGATG
   
Sequence Name 25-K033706-022-405-E03C-IN37
GenBank Accession LN497342 [GenBank]
Sequencing primer gene specific primer, IN37: 5'-ACATCTTCGTTTTCCTAGACCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K033706-022-405-E03C-IN37
TGTTGTCATATGCTTTTGGCTTTTTTGGCTTTCTCGACCCATTTTTCGAACCCTTTGGTC
GGCCACGTCCTCGCTTTCCTGGACCAGTACTAGGACCAGCACTCGCATCATAAGGTTGGT
TGTTGAATTGATCAAATGGTCTTCTGTTTTGTAGTGGTGAGACCATTACATGAGGAGGTG
CTGATGTTGCGTGGGCGTCTTCATTGACTTCATGAGATGCTTCTCGGTCTATTGGACTAG
TACTAGGACCGGCATCTCGGTCATAAGAGTCCTCAATGTTGAAGTTATCTAATAGCCAAA
GGGTTTGTGGCGGTGCATGAGGAGGTATTGATGTTGCGTGGGCTTCTTCATTAACAGCAT
CTGATCTTTCTCTGTGGACTGGACCAGTACTAGGACCAGCATCTCGGTCATAAGAGTCCT
CAATGTTGAAGTTATCTAATAGCCAAAGGGTTTGTGGCGGTGCATGAGGAGGTACTGATG
TTGCGTGGGCTTCTTCATTAACAGCATCAGATTTTTCTCGGTGGACTAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11