Sequences from confirmation amplicon


Confirmation sequences for line 406G11 and hit At1g17460 are shown.
Line ID 406G11
Locus At1g17460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6000845
Primers used for PCR NC63 - 8474, show primer details
   
Sequence Name 86-K101272-0022-406-G11C-8409
GenBank Accession LN514630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K101272-0022-406-G11C-8409
TTTAGGAATATCCCATTTTGATTCTTCTTTTGAGCCACGTTTAGTCCTTAGAAGTAGTTT
CACGACCAAACGTGGCTCTGAAAGTTTCTTGTAATTCTTTAATTGAAAGATTAGCCAAGC
TTATCTCGCCTGGTACTCTAGAAAAATCAGGTTTTGAGGCAGCAGTTGACAATGCAGGTT
CTTTAGGACTAGCTGTAGCATTTAAGACATCCGTAGATTGATTAATACTGCATCCATTTG
ATGAAAAAAGAATTGGTTCCTGCAGCAAAACTTTGGATGGGACTAGCTCCACATTCCCAG
ACAGCTCAGTAGAAATCTGAAATTTGGTATCCGGAAGAACAAAAAGCATCTGAGATCCAT
TCATCAATTCCTCTTTAGACTGCAAAAGTTTCAAAGGTGGAGTAAAATTGATGGCTATAA
CAGCCAACTGACCCATGATAGTTATACAGGAATAACAATATTAGAAAGTTCTGTTTCTAG
ATTTTGAAGCAAGAAAACAAACCTCATGTCGAGAGTTCAAATTTTCTGTATACGGTCCTG
CAGTTTCCGACTGAAAGAAGCCTGGATTGAAT
   
Sequence Name 86-K101278-0022-406-G11C-NC63
GenBank Accession LN514631 [GenBank]
Sequencing primer gene specific primer, NC63: 5'-TATTCAATCCAGGCTTCTTTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K101278-0022-406-G11C-NC63
AATTTGACTCTCGACTGAGGTTTGTTTTCTTGCTTCAAATCTAGAAACAGAACTTTCTAA
TATTGTTATTCCTGTATAACTATCATGGGTCAGTTGGCTGTTATAGCCATCAATTTTACT
CCACCTTTGAAACTTTTGCAGTCTAAAGAGGAATTGATGAATGGATCTCAGATGCTTTTT
GTTCTTCCGGATACCAAATTTCAGATTTCTACTGAGCTGTCTGGGAATGTGGAGCTAGTC
CCATCCAAAGTTTTGCTGCAGGAACCAATTCTTTTTTCATCAAATGGATGCAGTATTAAT
CAATCTACGGATGTCTTAAATGCTACAGCTAGTCCTAAAGAACCTGCATTGTCAACTGCT
GCCTCAAAACCTGATTTTTCTAGAGTACCAGGCGAGATAAGCTTGGCTAATCTTTCAATT
AAAGAATTACAAGAAACTTTCAGAGCCACGTTTGGTCGTGAAACTACTTCTAAGGACAAA
CGATGGCTCAAAAGAAGAATCAAAATGGGTATATTCCTAAAACTACATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
Primers used for PCR NC64 - 8474, show primer details
   
Sequence Name 87-K101272-0022-406-G11C-OriL
GenBank Accession LN514737 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101272-0022-406-G11C-OriL
TTTACAATTGAATATATCCTGACAATTGTACATATTGATTCATGTGTAGTTCCAACAACT
ACCCTGACAATTAATGATAGCAAACTGATTGGTGGAGATCAAGATGCAATTGATGCTTTT
AGCAAGGGTACAGTAGATGAAGAAACAGCTACTGAAAGCATAGATACACCAGCTAGTCCT
GACGGCATAAAGGGGCATTCAAATGATTTTGGGCATAGTCCTGTTGAAACTTTTGTGGAC
CATTATTCTGGAAACGAAGATTTTGAAGGTGAAGATGGGTCTGCAAAAAGAGTGAGGAAG
CCTACAAGGAGATATATTGAAGAAACCAATGAAAAACAACAAATTGACGGGTCAATGATC
CCTTCAAAAGATCCAAGCTCTATTCAAGCCGTTTCTTCAGAAGGGAGAGTTGTTGTCACC
AGGATGGTATCCCTTGCTGGTTCCAGAATTCAGGTTCCCTATGTTTCTCATGTAAGGAGA
AGTCGTCCAAGAGAAAACATAATGGCTCTGGGGGTATGTCTCTATTGTTCATGCTCTTCA
ATATCTTTATTAACCCCATTCCTTATCAA
   
Sequence Name 87-K101278-0022-406-G11C-NC64
GenBank Accession LN514738 [GenBank]
Sequencing primer gene specific primer, NC64: 5'-TAGCTCATATGATAAGGAATGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101278-0022-406-G11C-NC64
GACATAGAGACATACCCCAAGAGCCATTATGTTTTCTCTTGGACGACTTCTCCTTACATG
AGAAACATAGGGAACCTGAATTCTGGAACCAGCAAGGGATACCATCCTGGTGACAACAAC
TCTCCCTTCTGAAGAAACGGCTTGAATAGAGCTTGGATCTTTTGAAGGGATCATTGACCC
GTCAATTTGTTGTTTTTCATTGGTTTCTTCAATATATCTCCTTGTAGGCTTCCTCACTCT
TTTTGCAGACCCATCTTCACCTTCAAAATCTTCGTTTCCAGAATAATGGTCCACAAAAGT
TTCAACAGGACTATGCCCAAAATCATTTGAATGCCCCTTTATGCCGTCAGGACTAGCTGG
TGTATCTATGCTTTCAGTAGCTGTTTCTTCATCTACTGTACCCTTGCTAAAAGCATCAAT
TGCATCTTGATCTCCACCAATCAGTTTGCTATCATTAATTGTCAGGGTAGTTGTTGGAAC
TACACATGAATCAATATGTACAATTGTCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCA

Last Updated on 06.12.2018 16:11