Sequences from confirmation amplicon


Confirmation sequences for line 407D08 and hit At1g54870 are shown.
Line ID 407D08
Locus At1g54870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20459482
Primers used for PCR JG76 - 8474, show primer details
   
Sequence Name 69-K034069-022-407-D08C-JG76
GenBank Accession LN512138 [GenBank]
Sequencing primer gene specific primer, JG76: 5'-CTTCCTTCCTTCATATGCTTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K034069-022-407-D08C-JG76
CCACACGTAGGTTTGACTTCGTCACAACAAAATATCACACGTGTGCCACCTTGTGAGAAA
GAAGTAAGAAAAGATGTTTGTACGGAAGACTCGCTCAAGCCTAGGCTCATCAATCTCTTC
GATTGTGCTGCTCTCGTACTGCTCTGCTGCGTTATTGATCAAAACATCGATGCGGCCAAA
AGCATTAACGACCTCATCAACGACCCTTTTGCAGTTTTCGTCAAATCCTAAATCCGTTGG
AATGGCGATAGGTTCCTTGGAGTCCGAGGTTTTGACCTCCTTCAACATTTGTAGGGTCTC
TTGTGCATCTTTTTCTTCTTGACCCTTCACGTAAGTGAAAGCCACAGTAGCTCCTTCGGA
TGCAAAACAGTATCCCACGGCTCGACCAATCCCAGAGTCTCCACCAGTCCGGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTCAC
   
Sequence Name 53-K034069-022-407-D08C-oril
GenBank Accession LN508070 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K034069-022-407-D08C-oril
AAGCCATTTACAATTGAATATATCCCGGACTGGTGGAGACTCTGGGATTGGTCGAGCCGT
GGGATACTGTTTTGCATCCGAAGGAGCTACTGTGGCTTTCACTTACGTGAAGGGTCAAGA
AGAAAAAGATGCACAAGAGACCCTACAAATGTTGAAGGAGGTCAAAACCTCGGACTCCAA
GGAACCTATCGCCATTCCAACGGATTTAGGATTTGACGAAAACTGCAAAAGGGTCGTTGA
TGAGGTCGTTAATGCTTTTGGCCGCATCGATGTTTTGATCAATAACGCAGCAGAGCAGTA
CGAGAGCAGCACAATCGAAGAGATTGATGAGCCTAGGCTTGAGCGAGTCTTCCGTACAAA
CATCTTTTCTTACTTCTTTCTCACAAGGTGGCACACGTGTGATATTTTGTTGTGACGAAG
TCAAACCTACGTGTGGTTGTAATTGATATATTCCATTTTAATTTTGTTAGGCATGCGTTG
AAGCATATGAGGAAAGGAAGAAAGTACCAAATACGCACTTGAGACA
Primers used for PCR JG77 - 8474, show primer details
   
Sequence Name 70-K034069-022-407-D08C-JG77
GenBank Accession LN512324 [GenBank]
Sequencing primer gene specific primer, JG77: 5'-GTTTACCAACACGTGTATCCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K034069-022-407-D08C-JG77
CAGATCCTANTGTTGAGATATATTGCTACTTCAATGTAGCATGTTGTATATATGGCCATG
AATGTGTTGTGTCGAGTGTTCACTTCGTCAAGAGTCTTATCATCAAACGTTACGTTCTCG
TTTTCTCAAATTCCAAACAATACCAAGAAACCTCTACTTGAAAAGAGAAGATTATCGCCG
CGTGTGTGCCTAAGAGCGATGGCGTCTGAGAAACAAAAACAACATGCACAACCTGGCAAA
GAACATGTCATGGAATCAAGCCCACAATTCTCTAGCTCAGATTACCAACCTTCCAACAAG
CTTCGTGTAATTTTTTATAACCTTTAGTTTGAGATCAAAATATTACATAATTTCAAATCT
TTACGTACCATATTCATTTTTCGAGTACGTAAATTTGTATATGCATATTGGTATATATTT
CATAAGATAATTTGATCTCAATTCAAATTCACTCAATTAGCTAATTATTTTAAAGGATTT
GAGATTTTAATATTTCTTTAACAACATGGATGTAAATCAGGGTAAGGTGATTTTAATATT
TCTTTAACAACATGGATGTAAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 54-K034069-022-407-D08C-8409
GenBank Accession LN508395 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K034069-022-407-D08C-8409
ATTTACAATTGAATATATCCTGATTTACATCCATGTTGTTAAAGAAATATTAAAATCACC
TTACCCTGATTTACATCCATGTTGTTAAAGAAATATTAAAATCTCAAATCCTTTAAAATA
ATTAGCTAATTGAGTGAATTTGAATTGAGATCAAATTATCTTATGAAATATATACCAATA
TGCATATACAAATTTACGTACTCGAAAAATGAATATGGTACGTAAAGATTTGAAATTATG
TAATATTTTGATCTCAAACTAAAGGTTATAAAAAATTACACGAAGCTTGTTGGAAGGTTG
GTAATCTGAGCTAGAGAATTGTGGGCTTGATTCCATGACATGTTCTTTGCCAGGTTGTGC
ATGTTGTTTTTGTTTCTCAGACGCCATCGCTCTTAGGCACACACGCGGCGATAATCTTCT
CTTTTCAAGTAGAGGTTTCTTGGTATTGTTTGGAATTTGAGAAAACGAGAACGTAACGTT
TGATGATAAGACTCTTGACGAAGTGAACACTCGACACAACACATTCATGGCCATATATAC
AACATGCTACATTGAAGTAGCAATATATCTCAACATAGGATCTGACATGTGGCAGGTTGA
GGAACATGACATGGTCGGAGGAGGAT

Last Updated on 06.12.2018 16:11