Sequences from confirmation amplicon


Confirmation sequences for line 410B07 and hit At5g07620 are shown.
Line ID 410B07
Locus At5g07620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2408130
Primers used for PCR EQ53 - 8474, show primer details
   
Sequence Name 15-K103074-0022-410-B07C-EQ53
GenBank Accession LN492929 [GenBank]
Sequencing primer gene specific primer, EQ53: 5'-AGATAGAACTCATCATCGCCAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103074-0022-410-B07C-EQ53
CTCAGCATGATCACACCAAGACTATACACATCACTCTCTTTGCTCACGTCCTTCATCTTT
ATTAACTCAGGAGCTTTGTATCCCTCAGCTGCAGAAACATCAAGAATCTCTTGTCCTGCA
GACAGGTTCAAGAGCAAATGTAAACCGAAATCAGAGATTCTAGGTTCGAAACTCGAACTG
AGAAGAACGTTCTTGGACTTCAGATTGCCATGGACGATTGGTTTTTGCATTCCTGTATGA
AGATGATCAAGGGCTTTCGATATTCCAATTGTGATTCTTAAAATATTGATCCATTTTCTT
GATTCATCATCTCCACCTGTCAAAACAAAACAAGAATGTAATTTTCTTATTAGTCTAGAT
ATTTGCTGAGATTGATGTTCTTAGTGTCAAATTTTGTTCTTACTACACTAAAGAAGTGGA
AAATGACAGAAAATAAAACAAAAATAGTACCGACAAAAGACAAAGATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 16-K103074-0022-410-B07C-8409
GenBank Accession LN493389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103074-0022-410-B07C-8409
CTTTGTCTTTTGTCGGTACTATTTTTGTTTTATTTTCTGTCATTTTCCACTTCTTTAGTG
TAGTAAGAACAAAATTTGACACTAAGAACATCAATCTCAGCAAATATCTAGACTAATAAG
AAAATTACATTCTTGTTTTGTTTTGACAGGTGGAGATGATGAATCAAGAAAATGGATCAA
TATTTTAAGAATCACAATTGGAATATCGAAAGCCCTTGATCATCTTCATACAGGAATGCA
AAAACCAATCGTCCATGGCAATCTGAAGTCCAAGAACGTTCTTCTCAGTTCGAGTTTCGA
ACCTAGAATCTCTGATTTCGGTTTACATTTGCTCTTGAACCTGTCTGCAGGACAAGAGAT
TCTTGATGTTTCTGCAGCTGAGGGATACAAAGCTCCTGAGTTAATAAAGATGAAGGACGT
GAGCAAAGAGAGTGATGTGTATAGTCTTGGTGTGATCATGCTTGAGCTAGTCTCTGGTAA
AGAACCGATAAACGAGAATGCAACTGGCGATGATAGTTTCTATC

Last Updated on 06.12.2018 16:11