Sequences from confirmation amplicon


Confirmation sequences for line 413B10 and hit At3g53810 are shown.
Line ID 413B10
Locus At3g53810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19934898
Primers used for PCR EQ55 - 8474, show primer details
   
Sequence Name 20-K103075-0022-413-B10C-8409
GenBank Accession LN495170 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103075-0022-413-B10C-8409
GCCTTTACATTGAATATATCCTGGATCAATGGAGAAGGATGATACGTTGCCGTTTTGGGA
ATCTTTGAACCGGATTCGTTCAGTACAGAAGGCGTGACCGGTTTTTTGTACCGATGTGTT
GGTAAGCTTCAAGAGACCATTGGGTGTTACTGTTGCGAGACCTTGGAGTGAGATATCTGT
TAATGGAGGATGGAAACCATTGTAGGTGAAGTTGAGATTTTGAGAGGAAGAAATCATAAT
TTGACAGAGTAGGAAGAAGAAGAAGATGAGCTTGAGCTTGACGAACATGGCTGCTTCCGG
TTTAATCCGGTTTAGTTTTACTCTGTTTTGTTAAACGAAAGAGGAAAAGATTGAAGATAG
AAATTGAAGTTATTAAAAGAGGTGAAGAGAAGGATAAGAAAAAAAAGTCGTGGTAGTGTT
TCTTTGAGGCCACGCTTTGTGAGATTTGAATAATATGTCAGTGGTTGGTGAAGTATTAAT
CAAGATTATATAAGCTTGAATTAAATAGAAGTTAATTTCATTCTATGATTAATTGGTTTT
ATAAGGAGGAAGTGTATATACAAAAAAAAACCACACTTTGATTTTGACTGATGCATTAAT
CCAGTTTGTCTGCATGTGACTTCTAGTGGTCAATGGTCAATGA
   
Sequence Name 19-K103075-0022-413-B10C-EQ55
GenBank Accession LN494718 [GenBank]
Sequencing primer gene specific primer, EQ55: 5'-CTCATTGACCATTGACCACTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103075-0022-413-B10C-EQ55
TATGCATCAGTCAAAATCAAAGTGTGGTTTTTTTTTGTATATACACTTCCTCCTTATAAA
ACCAATTAATCATAGAATGAAATTAACTTCTATTTAATTCAAGCTTATATAATCTTGATT
AATACTTCACCAACCACTGACATATTATTCAAATCTCACAAAGCGTGGCCTCAAAGAAAC
ACTACCACGACTTTTTTTTCTTATCCTTCTCTTCACCTCTTTTAATAACTTCAATTTCTA
TCTTCAATCTTTTCCTCTTTCGTTTAACAAAACAGAGTAAAACTAAACCGGATTAAACCG
GAAGCAGCCATGTTCGTCAAGCTCAAGCTCATCTTCTTCTTCTTCCTACTCTGTCAAATT
ATGATTTCTTCCTCTCAAAATCTCAACTTCACCTACAATGGTTTCCATCCTCCATTAACA
GATATCTCACTCCAAGGTCTCGCAACAGTAACACCCAATGGTCTCTTGAAGCTTACCAAC
ACATCGGTACAAAAAACCGGTCACGCCTTCTGTACTGAACGAATCCGGTTCAAAGATTCC
CAAAACGGCAACGTATCATCCTTCTCCATTGATCCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCACGGTT
Primers used for PCR KF65 - 8474, show primer details
   
Sequence Name 52-K118780-0022-413-B10C-KF65
GenBank Accession LN507967 [GenBank]
Sequencing primer gene specific primer, KF65: 5'-ATCAAAGGCATACCGATTTTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118780-0022-413-B10C-KF65
TCGAGGAAGCTTCGGGAGTTTCGATAACGAGAGCATCGGAGCTTCTCCGTTTAACCGGAA
ACTCCATCCGACTAAGAAATGCTCCGAGAGAACTGAACCAGTCGCAGATGAGAAACCTAC
ATACATATCTTCTAGAAGAATCGAAGAAAGATCTCTCACATACGAAACTAGAGGTTTTCT
AGGTTTATCAGAATCAAACGGAGCCACGGTGACATCAATTCGATGACTACGATTATCGTA
ATCGATCCAAACTTGGATCCGTTTGCGACTAATCAAACTCAGATTCTGAAACTTATCGTG
ATCATCCCTATATCCCGCCGTCGAGTAATTCGCCGATCGTAAACCGTTGAGATCGATTCC
GACGTGATTATCGTTTGGATCTCCAAACTCGCTACTCTGGATTGTATCGAATTCGACGGC
AAAGATGTGATTCGTGTCGTTACCATTGTTAGAGATGTTGAAGAGACCGATGTATTGACT
AGGAAGTGCAAAAGGAAGACCTAGCGTTGGAGCTACAACGAAGGCGATTCCATGGCCGCT
TAGTGTTGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCATAAATGTAGATATCCG
   
Sequence Name 12-K118779-0022-413-B10C-8409
GenBank Accession LN491805 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118779-0022-413-B10C-8409
CATTACATTGAATATATCCCAACACTAAGCGGCCATGGAATCGCCTTCGTTGTAGCTCCA
ACGCTAGGTCTTCCTTTTGCACTTCCTAGTCAATACATCGGTCTCTTCAACATCTCTAAC
AATGGTAACGACACGAATCACATCTTTGCCGTCGAATTCGATACAATCCAGAGTAGCGAG
TTTGGAGATCCAAACGATAATCACGTCGGAATCGATCTCAACGGTTTACGATCGGCGAAT
TACTCGACGGCGGGATATAGGGATGATCACGATAAGTTTCAGAATCTGAGTTTGATTAGT
CGCAAACGGATCCAAGTTTGGATCGATTACGATAATCGTAGTCATCGAATTGATGTCACC
GTGGCTCCGTTTGATTCTGATAAACCTAGAAAACCTCTAGTTTCGTATGTGAGAGATCTT
TCTTCGATTCTTCTAGAAGATATGTATGTAGGTTTCTCATCTGCGACTGGTTCAGTTCTC
TCGGAGCATTTCTTAGTCGGATGGAGTTTCCGGTTAAACGGAGAAGCTCCGATGCTCTCG
TTATCGAAACTCCCGAAGCTTCCTCGATTTGAGCCTAGAAGAATCTCTGAATTCTACAAA
ATC

Last Updated on 06.12.2018 16:11