Sequences from confirmation amplicon


Confirmation sequences for line 413C02 and hit At4g16070 are shown.
Line ID 413C02
Locus At4g16070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9098214
Primers used for PCR 22uj - 8474, show primer details
   
Sequence Name 23-K117118-0022-413-C02C-22uj
GenBank Accession LN496723 [GenBank]
Sequencing primer gene specific primer, 22uj: 5'-TGAGAATTGCACTGTTGAAATAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117118-0022-413-C02C-22uj
CTTTGTTCAGATTGTAGGTCATTCTCTTGGAGGTGGGACAGCTTCACTTTTAACGTATAT
TCTTCGGGAGCAAAAAGAGTTTGCCTCGGCTACCTGTTTCACTTTTGCACCAGGTACACC
CAATTTAATGATTAATGGTGGTATTTTTTTGTCTTCAGTTATCTGCTCCTCTCCCTTAAT
TGTGATGCTCAACTCTTTATATGTCAATTAGTTCTCTGGGTCAAAAATTGTTAATATGCA
CTGAATTTCAAATTGAACAACTCGCAGCTGCTTGTATGACTTGGGATTTAGCAGAATCAG
GCAAGCACTTCATTACTACTATTATCAATGGATCTGATCTAGTCCCAACATTCTCTGCTT
CTTCAGTCGATGACCTTCGTTCTGAGGTATATGACCTGCTTTGCTTATATGTGAAGCTAC
ATTCACAGGATTTATCTCTGCCACCATTAGGAGAAATTTTCCTGATTCTGTAGTGGTACA
ATATATTGCTAACCTTGGTATATCAAATAGTGGATACAAGCCTGCAATTTTAACTTGGTA
CTTCTGTAGAGAGAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 01-K117117-0022-413-C02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117117-0022-413-C02C-8409
GGCAAGCACTTCATGTACTACTATTATCTAGGGCATCTGATCTAATGCCA

Last Updated on 06.12.2018 16:11