Sequences from confirmation amplicon


Confirmation sequences for line 414B10 and hit At1g72850 are shown.
Line ID 414B10
Locus At1g72850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27414922
Primers used for PCR 32lq - 8474, show primer details
   
Sequence Name 56-K113947-0022-414-B10C-32lq
GenBank Accession LN509171 [GenBank]
Sequencing primer gene specific primer, 32lq: 5'-AAGAAAATCTCAGATAAACCGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113947-0022-414-B10C-32lq
CATTAATGACAACAGATCTACAACTTGTTGACATTAACAGATCATCACTTTTTTAATATC
TTCTTGAGTGATTCATTCACTTATTAATCATCACTCAAGAGAATAAATGATGTGATTTTC
TCTTTATCTTCTTCTTTTTTTCCTGCTCTTGTGTATCTCTTGCTCGTGATTTCCATAATA
GCTTTCTCTTGGTCTCCCTCCAGTTGTTGCAGTTCCTTCTCCCAACGCTCTTCATCCTTG
CCTCGTAGAGAAGACCCGAGGATTTTAAGTGTCAGAGGAAGGAAACCCGATATATGGACA
GCGCGAAGGGATAGTGATTCAAAACTAGTGGGAGGGTGTTGCTGGTCAAAAGCAGAGTGA
GAAAATAGTTGAAGAGCTTCGTCGTATCTCAGAGACTGAACTTCATAAGCATGCTCCACA
CCAGACTCAACTAACAGGTTCCTGTCCTGTGTAACAAAGATCACTCTACTTCCTGGAATA
AACCAGTGAGCAAGGTAATTACCAACGCAATCAATCACGATCAGAATAATCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCG
   
Sequence Name 28-K113946-0022-414-B10C-8409
GenBank Accession LN498936 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K113946-0022-414-B10C-8409
TTTCATTGAATATATCCTGATTATTCTGATCGTGATTGATTGCGTTGGTAATTACCTTGC
TCACTGGTTTATTCCAGGAAGTAGAGTGATCTTTGTTACACAGGACAGGAACCTGTTAGT
TGAGTCTGGTGTGGAGCATGCTTATGAAGTTCAGTCTCTGAGATACGACGAAGCTCTTCA
ACTATTTTCTCACTCTGCTTTTGACCAGCAACACCCTCCCACTAGTTTTGAATCACTATC
CCTTCGCGCTGTCCATATATCGGGTTTCCTTCCTCTGACACTTAAAATCCTCGGGTCTTC
TCTACGAGGCAAGGATGAAGAGCGTTGGGAGAAGGAACTGCAACAACTGGAGGGAGACCA
AGAGAAAGCTATTATGGAAATCACGAGCAAGAGATACACAAGAGCAGGAAAAAAAGAAGA
AGATAAAGAGAAAATCACATCATTTATTCTCTTGAGTGATGATTAATAAGTGAATGAATC
ACTCAAGAAGATATTAAAAAAGTGATGATCTGTTAATGTCAACAAGTTGTAGATCTGTTG
TCATTAATGTATAANATGACATTATCACTGATGAACATTGCGGTT

Last Updated on 06.12.2018 16:11