Sequences from confirmation amplicon


Confirmation sequences for line 414D07 and hit At3g27925 are shown.
Line ID 414D07
Locus At3g27925 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10368826
Primers used for PCR HM19 - 8474, show primer details
   
Sequence Name 19-K028919-022-414-D07C-8409
GenBank Accession LN494627 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K028919-022-414-D07C-8409
CATTTACAATTGAATATATCCTTCACTCTCCTCCTTCACTCTCCTCCTTCTTCTCACCTC
TCCTTCTTCAATCTCTCTTCTTCACGATCCTCTCCAATTTCTCTTTACCCAATCCGATCC
AAACGCTATTTCCGAATCCTCTCGAAGCTTTCACTAAACGACAACAACAGAGACGACGAT
GATGATACTCTTCATTTTACTCCGTTCTCCGCCGTTAAACCTTTCTTCCTCCTCTGTACC
TCCGTCGCGTTATCGTTTTCACTCTTCGCCGCTTCTCCCGCCGTTGAATCTGCCTCGGCG
TTTGTAGTTAGTACTCCGAAGAAACTTCAAACTGATGAGCTCGCGACGGTTCGTTTGTTT
CAAGAGAACACTCCTTCCGTTGTTTACATCACTAATCTCGCCGTGAGGTATCTGAATTTG
ATTTCTCTCTGGTGAATTTCGTTTTGAATTTCTTCTCTCCGTGATTTGAGGATTGGTCTA
ATGTTGTTTGAATTAGGCAGGACGCGTTTACATTGGACGTTCTTGAGGTACCTCAAGGCT
CTGGTTCAGGATTTGTGTGGGATAAGCA
   
Sequence Name 20-K028919-022-414-D07C-HM19
GenBank Accession LN495079 [GenBank]
Sequencing primer gene specific primer, HM19: 5'-AATATGACCTTGCTTATCCCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K028919-022-414-D07C-HM19
GTAAACGCGNTCCTGCCTAATTCAAACAACATTAGACCAATCCTCAAATCACGGAGAGAA
GAAATTCAAAACGAAATTCACCAGAGAGAAATCAAATTCAGATACCTCACGGCGAGATTA
GTGATGTAAACAACGGAAGGAGTGTTCTCTTGAAACAAACGAACCGTCGCGAGCTCATCA
GTTTGAAGTTTCTTCGGAGTACTAACTACAAACGCCGAGGCAGATTCAACGGCGGGAGAA
GCGGCGAAGAGTGAAAACGATAACGCGACGGAGGTACAGAGGAGGAAGAAAGGTTTAACG
GCGGAGAACGGAGTAAAATGAAGAGTATCATCATCGTCGTCTCTGTTGTTGTCGTTTAGT
GAAAGCTTCGAGAGGATTCGGAAATAGCGTTTGGATCGGATTGGGTAAAGAGAAATTGGA
GAGGATCGTGAAGAAGAGAGATTGAAGAAGGAGAGGTGAGAAGAAGGAGGAGAGTGAAGG
AGGAGAGTGAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11