Sequences from confirmation amplicon


Confirmation sequences for line 414F05 and hit At5g67250 are shown.
Line ID 414F05
Locus At5g67250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26833284
Primers used for PCR KS03 - 8474, show primer details
   
Sequence Name 51-K023492-022-414-F05C-8409
GenBank Accession LN507371 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K023492-022-414-F05C-8409
CCGGACATGAAGCCATTTACAATTGAAATCGAAACCATTCGGTTGGTGATGGGCCAAGCG
CCGTCGTCTACGGCGGAATCGAACGGACGAGAGTTAGATTTGAGGTTATGGTCTCCGGTG
ATTGTTGCTGGAGGTGAATCGATGGCTGTGGGGAATGTTGTGGATCGGGATTTCACCGGA
GATCTGCCGGATGAGTGTTTAGCTCATGTGTTTCAGTTTCTTGGAGCTGGAGATCGGAAG
CGATGCTCTCTTGTTTGTAAACGGTGGTTGCTCGTCGACGGTCAGAGTCGTCATCGGTTA
TCTCTAGATGCTAAGGATGAGATCTCCTCTTTCCTTACCTCTATGTTCAATCGATTCGAT
TCGGTGACGAAGCTTGCTCTCCGATGTGATCGGAAGTCTGTTAGCTTGAGCGATGAAGCT
TTGGCTATGATCTCTGTTCGTTGTTTGAATCTGACTCGTGT
   
Sequence Name 52-K023492-022-414-F05C-KS03
GenBank Accession LN507688 [GenBank]
Sequencing primer gene specific primer, KS03: 5'-GAAGCTTAACACGAGTCAGATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K023492-022-414-F05C-KS03
ACAACGAACAGAGATCATAGCCAAAGCTTCATCGCTCAAGCTAACAGACTTCCGATCACA
TCGGAGAGCAAGCTTCGTCACCGAATCGAATCGATTGAACATAGAGGTAAGGAAAGAGGA
GATCTCATCCTTAGCATCTAGAGATAACCGATGACGACTCTGACCGTCGACGAGCAACCA
CCGTTTACAAACAAGAGAGCATCGCTTCCGATCTCCAGCTCCAAGAAACTGAAACACATG
AGCTAAACACTCATCCGGCAGATCTCCGGTGAAATCCCGATCCACAACATTCCCCACAGC
CATCGATTCACCTCCAGCAACAATCACCGGAGACCATAACCTCAAATCTAACTCTCGTCC
GTTCGATTCCGCCGTAGACGACGGCGCTTGGCCCATCACCAACCGAATGGTTTCGATTTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATGGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TACAT

Last Updated on 06.12.2018 16:11