Sequences from confirmation amplicon


Confirmation sequences for line 421B06 and hit At5g44790 are shown.
Line ID 421B06
Locus At5g44790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18080225
Primers used for PCR PE93 - 8474, show primer details
   
Sequence Name 17-K022562-022-421-B06C-8409
GenBank Accession LN493684 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K022562-022-421-B06C-8409
AGATTTCCCGGACATGAAGCCATTTCTTTTTCTTCGAGTATTTTATATAGGAGCTGAAGT
AAAAAAAGAAAAGCATTTTATAGGAGAAATCTATTATTACTATGTACGTATGTAATTAAA
GGAAATATTAATAGTACTTGTTCAAGAGTTAACAAAAAATCTGGAAATATTAATAGAGTT
TTAAAATATCTGAAAACATTTAAAAAAAAAACTGAAAATATTAATAGATTTTTAATGAAA
AATCTAGAGCACTAGAGCTTTGATCTATACTTTTTTCATTAAATATTCTTAGAAACATGT
TAAATGACATTTGTTAATGGTTTTTTTTACAGCATTTGTTAATGATTTTGATTGGTCTTG
TTGATTTTTTAATTATTGCATACAGTTGTTCAAAACGTGTATGTTAAATAATTATTGTTT
GAAGTCGTCGTCTACTAATTAATTTGATTCCAAAATTAAACAACCTAGTGTAGTCATAGA
CGAAGGAAAAAAAATACGATAGTAGTAGACTAGTAGTAAATGTTTA
   
Sequence Name 18-K022562-022-421-B06C-PE93
GenBank Accession LN494132 [GenBank]
Sequencing primer gene specific primer, PE93: 5'-CCATGTGCCCATAAACATTTACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K022562-022-421-B06C-PE93
TAGTCTACTACTATCGTATTTTTTTTCCTTCGTCTATGACTACACTAGGTTGTTTAATTT
TGGAATCAAATTAATTAGTAGACGACGACTTCAAACAATAATTATTTAACATACACGTTT
TGAACAACTGTATGCAATAATTAAAAAATCAACAAGACCAATCAAAATCATTAACAAATG
CTGTAAAAAAAACCATTAACAAATGTCATTTAACATGTTTCTAAGAATATTTAATGAAAA
AAGTATAGATCAAAGCTCTAGTGCTCTAGATTTTTCATTAAAAATCTATTAATATTTTCA
GTTTTTTTTTTAAATGTTTTCAGATATTTTAAAACTCTATTAATATTTCCAGATTTTTTG
TTAACTCTTGAACAAGTACTATTAATATTTCCTTTAATTACATACGTACATAGTAATAAT
AGATTTCTCCTATAAAATGCTTTTCTTTTTTTACTTCAGCTCCTATATAAAATACTCGAA
GAAAAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
Primers used for PCR FN14 - 8474, show primer details
   
Sequence Name 55-K032008-022-421-B06C-8409
GenBank Accession LN508709 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K032008-022-421-B06C-8409
AGCATTTTTTCATGTTTTCATTTTTGTTTTTTTTTTTAAAATTTTTTTTTCTTCAAAATT
CCTCCATTGTTATTACATAAAGGAAAAAAGAAAGGTCTCTGAGATTTTCAAATCACCATC
ATCATCATCGTTGAAGAAGACGAAGAAGATACCTTCCTCTTTATCTTCTCCGATTTCTCA
ACCTTCCCGTCTGCTTGTCGTAAAGAAATGGCGCCGAGTAGACGGGATTTACAGCTTACT
CCGGTCACCGGAGGTTCTTCTTCCCAGATCAGTGATATGGAAGAAGTTGGTCTTCTCGAT
TCGTATCACAACGAAGCGAACGCCGATGATATTTTGACTAAA
   
Sequence Name 56-K032008-022-421-B06C-FN14
GenBank Accession LN509006 [GenBank]
Sequencing primer gene specific primer, FN14: 5'-ATTTTAGTCAAAATATCATCGGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032008-022-421-B06C-FN14
ACTTCTTCCTATCACTGATCTGGGAAGAAGAACCTCCGGTGACCGGAGTAAGCTGTAAAT
CCCGTCTACTCGGCGCCATTTCTTTACGACAAGCAGACGGGAAGGTTGAGAAATCGGAGA
AGATAAAGAGGAAGGTATCTTCTTCGTCTTCTTCAACGATGATGATGATGGTGATTTGAA
AATCTCAGAGACCTTTCTTTTTTCCTTTATGTAATAACAATGGAGGAATTTTTGAAGAAA
AAAAAATTTTAAAAAAAAAAACAAAAATGAAAACATTGAAAAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11