Sequences from confirmation amplicon


Confirmation sequences for line 422C08 and hit At5g16560 are shown.
Line ID 422C08
Locus At5g16560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5407665
Primers used for PCR NJ08 - 8474, show primer details
   
Sequence Name 60-K036619-022-422-C08C-NJ08
GenBank Accession LN510189 [GenBank]
Sequencing primer gene specific primer, NJ08: 5'-GGAGTAACTCTTCACGGTACGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K036619-022-422-C08C-NJ08
ACGATAAGTATATATAAACATTAGGTAAGAATAAGATTGACGAAATTTGAGATAGGGTTT
AGGGTTCTCTCACCTAATTGTTCCTACTTCCTACCAAGTAATTCATACGACAATTGTTTG
GTTTATCCCACATCTATCCATTTTCTTCTAACAAAAATAGTTTTTAGATGATATTTTAAG
TTTGTTTTCGTTCAGTTTAAGGGGAAAAAATTTGAGTGATGGTTTTGGTTACAATGAGAT
GAAATTGGTTAGGGTTTATGGCTGATGAACTTTAATTATATGTACCAAGTTTTTTTTTTT
AATTATAATTTGGTTTGGGTTCTTAAAATTCATCTAGGAAAACTAGGATAAACAAATTTT
GCTTCATAAGTATGACATGTAAATTGTGGTCATATAGCAAGTTGCAAAAAAACGCAATTT
GTGTGTAGTGATTTTTATTTTATTTTTAGGGCATCTATACATCAATAAATACAATTTGTC
CTTAATTTGTTTTCTTTCTTTTCTTAAATCATAAAATAAAAAGACATGGATGCCATGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAAAAAAAAAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACAATCCG
TCGTATTTATAGGCAAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTC
   
Sequence Name 59-K036619-022-422-C08C-8409
GenBank Accession LN509926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K036619-022-422-C08C-8409
GAGCCATTTACATTGAAATATCATGGCATCCATGTCTCTCTATTTTATGATTTAAGAAAA
GAAAGAAAACAAATTAAGGACAAATTGTATTTATTGATGTATAGATGCCCTAAAAATAAA
ATAAAAATCACTACACACAAATTGCGTTTTTTTGCAACTTGCTATATGACCACAATCTAC
ATGTCATACTTATGAAGCAAAATTCGTTTATCCTAGTCTTCCTAGATGAATCTTAAGAAC
CCAAACCAAATTATAATTAAAAAAAAAAACTTGGTACATATAATTAAAGTTCATCACCCA
TAAACCCTAACCAATTTCATCTCATTGTAACCAAAACCATCACTCAAATTTTTTCCCCTT
AAACTGAACGAAAACAAACTTAAAATATCATCTAAAAACTATTTTTGTTAAAAAAAAATG
GATAGATGTGGGATAAACCAAACAATTGTCGTATGAATTACTTGGTAGGAAGTAGGAACA
ATTAGGTGAAAAAACCCTAAACCCTATCTCAAATTTCGTCAATCTTATTCTTACCTAAGG
TTTATATATACTTATCGTGTGTATAAAACATGTACGTATATACGTAAACGTACCGTG

Last Updated on 06.12.2018 16:11