Sequences from confirmation amplicon


Confirmation sequences for line 423F02 and hit At3g03120 are shown.
Line ID 423F02
Locus At3g03120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:717124
Primers used for PCR JU34 - 8474, show primer details
   
Sequence Name 03-K028094-022-423-F02C-8409
GenBank Accession LN487345 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028094-022-423-F02C-8409
ATTGCGGACCAAGTGGAATCTCTCTATACTGTACATAAGGAATCCATTGTACCGAAAACG
CAACCTTTGCTTTCTTGGGAAACTCTCTGCAATTCCTGGAGAAATCGACCATCATCATCT
TAGCTTTGATTCTCTCTGTGTTTCTTCACTTTCAATTTCTGTTTTGGTTTATCGATAAGA
GAAAGTAAAATTCGTCTGTGGGGGTTTAAGCTTTTTTGGGGTGAGAGATGGGTCAAACTT
TTCGCAAGCTTTTCGATACCTTCTTCGGCAACCAGGAGATGAGGGTACTCTCCTTCTCTC
CATCTTCTGTATTTGATTTCTGTATTTGATCGTTTACTGGACGGAATCGCGTGTTACCCA
TCGTTAATCGTTATACTCGCTTTCATTGTTTTGACGACACTCGGATTTTGAAATAATATG
AAAAAGTGTTTGATTTGTGCTAATCATTGATGTATCCTGGGATTTATTATATGTTCAAAG
AGTTACTTTTTAGGTCCGGTATATATGCTTGTAATCTAAACTCTTGCTAGTACTTTTAGG
TACCCTGAAGATTCAAGTTCCCTTCTACTATAGTATAGTACCTACACTGTTTAGAATCTT
GATGTAGCCAATCCACTTTCATAGACAGTTGGGTTACTTGTGTATCCATGAAATCATTCA
CACAAGTTAGCTAGTCCAGCTTTTGTTTTGTGTATTGAC
   
Sequence Name 03-K028094-022-423-F02C-JU34
GenBank Accession LN487346 [GenBank]
Sequencing primer gene specific primer, JU34: 5'-CATCATATCATGCACAGTGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028094-022-423-F02C-ju34
CATAATCTGTAACAGCACTTAATATCTAGAGTTATTGCACTTGATAGCTAATAAGCTGGG
TCAATACACAAAACAAAAGCTGGACTAGCTAACTTGTGTGAATGATTTCATGGATACACA
AGTAACCCAACTGTCTATGAAAGTGGATTGGCTACATCAAGATTCTAAACAGTGTAGGTA
CTATACTATAGTAGAAGGGAACTTGAATCTTCAGGGTACCTAAAAGTACTAGCAAGAGTT
TAGATTACAAGCATATATACCGGACCTAAAAAGTAACTCTTTGAACATATAATAAATCCC
AGGATACATCAATGATTAGCACAAATCAAACACTTTTTCATATTATTTCAAAATCCGAGT
GTCGTCAAAACAATGAAAGCGAGTATAACGATTAACGATGGGTAACACGCGATTCCGTCC
AGTAAACGATCAAATACAGAAATCAAATACAGAAGATGGAGAGAAGGAGAGTACCCTCAT
CTCCTGGTTGCCGAAGAAGGTATCGAAAAGCTTGCGAAAAGTTTGACCCATCTCTCACCC
CAAAAAAGCTTAAACCCCCACAGACGAATTTTACTTTCTCTTATCGATAAACCAAAACAG
AAATTGAAAGTGAAGAAACACAGAGAGAATCAAAGCTAAGATGATGATGGTCGATTTCTC
CAGGAATTGCAGAGAGTTTCCCAAGAAAGCAAAGGTTGCGTTTTCGGTACAATGGATTCC
TTATGTACAGTATAGAGAGATTCCACTTGGTCCGCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTT

Last Updated on 06.12.2018 16:11