Sequences from confirmation amplicon


Confirmation sequences for line 425D08 and hit F16D14 are shown.
Line ID 425D08
Locus F16D14 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13353808
Primers used for PCR DC79 - 8474, show primer details
   
Sequence Name 86-K034065-022-425-D08C-DC79
GenBank Accession LN514611 [GenBank]
Sequencing primer gene specific primer, DC79: 5'-GTGTTTGGAGCTAGCAACTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K034065-022-425-D08C-DC79
ATCTCCGTGATCATGACCGATGTGACGCCGTACGAACCATCTCTTACGAGGCTCACTCTC
GTCTCCATGATCCTATTTACGGCTGCGTCTCCCAAATCTTCTCCCTCCAACAACAGGTAT
TATACTTTTTCTTCGGAAATGTATACATAATTTTTTTTTGCTTTTGCTTCATCATCTCTA
GTGTACTTACAAGTAACTTCACTGACTTTGTTCTTAAGGTTGTTAGTTTACAAGCACAAG
TGGTTCTACTTAGAGAAGAAGCTTCGAGAAGGTTCCCTCAAGATGATCCTGGTTGTAATA
TGAAGCAACAAGAAAAGGTTCTTGCTCAACAAATGCCACAAGATCTTCACAATTGGTTTC
ACCAAGGAATCTTGGACTCAGACTTCGACCCAATGAGTGGTACTACACGCGACAATGAGC
GGTCCATGGATCATAATGAGTCTCCTTGTAGCTCAAATGAATCACTTAATTACTACCAGG
AGGTCAATTTTCCTTGGTCTGTTTGAGTACAAGTGACTACTTTGTCAGAGATGGAACCTT
ACAATCTTTCTATTGTATCGACTTTTCGACATTTTTCATAGTAGAGATCGAATCATGCAA
CCATTTTCTCTTTGAGAATAACCACTTCTTCGATGATCTTTCATAGGTACAACATCAGAT
CCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTG
CGAAGGATAGTGGGATTGTGCAATGAGTATGATGGTCAATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
   
Sequence Name 19-K034065-022-425-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K034065-022-425-D08C-8409
CGTCACATCGGTCATGATCAGGGAGATGAGAAATGAGCTTAGAGAAGTTGCTA

Last Updated on 06.12.2018 16:11