Sequences from confirmation amplicon


Confirmation sequences for line 428A06 and hit At1g08540 are shown.
Line ID 428A06
Locus At1g08540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2705300
Primers used for PCR IM08 - 8474, show primer details
   
Sequence Name 29-K032519-022-428-A06C-8409
GenBank Accession LN499177 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032519-022-428-A06C-8409
CCATTTACAATTGAATTATCCTGAGTATGATATATTGGCTAGAAAGCACAAAGAAGCGAT
CTTGGATTCTACCACTAACCACTAATTTCGTCATTCTCAAACATCTAAAGATTTTGTAGA
CATCATTTTCGCCTAGAATCTAGAGTCCTACGAAGGGTGAGTGTTTGTGATGTCTCAGAC
GCGATGTAGAAAAAGGGAGGTGGTTAGTTTAGAATTTGGAATATTGGTGACAAACCTGTA
TTCCTGCCGACAGCTCATGTTCTTCTCTGACAGTGAGAAGCTTGGAACTGCTTGTTGTCA
TTCTTAGATAACGCAAAGGATCATTATGATCAACTTCCTGCGCAACAAGACGTTTCTTTT
TAGGGCTCGAACCAGTCTTCACAGACGGAATACCTGATGCAGTTTTCTCTAACCCTT
   
Sequence Name 29-K032519-022-428-A06C-IM08
GenBank Accession LN499178 [GenBank]
Sequencing primer gene specific primer, IM08: 5'-AAAGGGTTAGAGAAAACTGCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032519-022-428-A06C-IM08
AACGTCTTGTTGCGCAGGAAGTTGATCATAATGATCCTTTGCGTTATCTAAGAATGACAA
CAAGCAGTTCCAAGCTTCTCACTGTCAGAGAAGAACATGAGCTGTCGGCAGGAATACAGG
TTTGTCACCAATATTCCAAATTCTAAACTAACCACCTCCCTTTTTCTACATCGCGTCTGA
GACATCACAAACACTCACCCTTCGTAGGACTCTAGATTCTAGGCGAAAATGATGTCTACA
AAATCTTTAGATGTTTGAGAATGACGAAATTAGTGGTTAGTGGTAGAATCCAAGATCGCT
TCTTTGTGCTTTCTAGCCAATATATCATACTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAT
GGG

Last Updated on 06.12.2018 16:11