Sequences from confirmation amplicon


Confirmation sequences for line 430D12 and hit At1g26790 are shown.
Line ID 430D12
Locus At1g26790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9275229
Primers used for PCR CP46 - 8474, show primer details
   
Sequence Name 09-K031138-022-430-D12C-8409
GenBank Accession LN490057 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K031138-022-430-D12C-8409
AGAGCCACAAGCCATAAAGACAGAGAAAATGGAGAAATTACACGTCAGCTTAGAGAGGGA
CATGAAGAGATGAGAGAGCCACATGTTGCAAAAAAGTGGGCTTTTTGCTATCTCTTTTCA
TGCTCTTTTTGATCTCTCTTTCAGTGCCACTAAAAAGATTTGCCTCCTTTATTGTCAGCC
ACGTCTCATTAAAATGTACAACTTCTTTTGGAATATTCATACAGAAAATTAGAAATACAA
TAATGCGAAAACATTTACAAAAATGCCAGCTGCCATACATCAGTTCAAAATGCTATATTA
CAGATGTTCCAATGTGGTTTTGTGAATGAGTACAAAATATTTTGGTTTCTATACGAATAA
CCAAAATATTGACTACTAAAAAAATTGTTGCTAGGGTAAGCAAAATCAGATATTACAATA
TGAATAACCAAAACATCGATTATAAAAACTAATTTCTATACAAATAACTAAAATATCGAT
AACTATTTTCTCCTTGTCATCCCTAGCGACGAGACAACAATCATCATTCTCCTCGAGATG
ATATCAAGATTGCGGTGGTTAAAATCTTGGTAAAGTGCTATGTTTACCATGTTGGACCAT
TTATTTTTACTCTTACTCTATATTATTTATCTAATAGGAGAAAAATTTACAACGTTGGTA
ATTTAGCTTCTCACATGCGTGATCGTGAGATACAAACACATTGTTGTGCACGATGTGTTT
GA
   
Sequence Name 44-K031138-022-430-D12C-CP46
GenBank Accession LN505072 [GenBank]
Sequencing primer gene specific primer, CP46: 5'-CACTCTAAACCAGCTTCAAACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K031138-022-430-D12C-CP46
ATCCGCATGTGAGAAGCTAATTACCAACGTTGTAAATTTTTCTCCTATTAGATAAATAAT
ATAGAGTAAGAGTAAAAATAAATGGTCCAACATGGTAAACATAGCACTTTACCAAGATTT
TAACCACCGCAATCTTGATATCATCTCGAGGAGAATGATGATTGTTGTCTCGTCGCTAGG
GATGACAAGGAGAAAATAGTTATCGATATTTTAGTTATTTGTATAGAAATTAGTTTTTAT
AATCGATGTTTTGGTTATTCATATTGTAATATCTGATTTTGCTTACCCTAGCAACAATTT
TTTTAGTAGTCAATATTTTGGTTATTCGTATAGAAACCAAAATATTTTGTACTCATTCAC
AAAACCACATTGGAACATCTGTAATATAGCATTTTGAACTGATGTATGGCAGCTGGCATT
TTTGTAAATGTTTTCGCATTATTGTATTTCTAATTTTCTGTATGAATATTCCAAAAGAAG
TTGTACATTTTAATGAGACGTGGCTGACAATAAAGGAGGCAAATCTTTTTAGTGGCACTG
AAAGAGAGATCAAAAAGAGCATGAAAAGAGATAGCAAAAAGCCCACTTTTTTGCAACATG
TGGCTCTCTCATCTCTTCATGTCCCTCTCTAAGCTGACGTGTAATTTCTCCATTTTCTCT
GTCTTTATGGCTTGTGGCTCTGTGTGATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATN
TCGACGTGTCTAC

Last Updated on 06.12.2018 16:11