Sequences from confirmation amplicon


Confirmation sequences for line 430F02 and hit At5g19620 are shown.
Line ID 430F02
Locus At5g19620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6623403
Primers used for PCR KI01 - 8474, show primer details
   
Sequence Name 09-K026766-022-430-F02C-8409
GenBank Accession LN490025 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K026766-022-430-F02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACTCACCAATCTCCAATCCTGCTCTAAAACCTT
CGTCTCTCACCTCTCAAATACTCGTAACTCACTAAACCAAATGCACCAGTCGCTAAAGAA
TCGCCACACTCCTCCTCCACGTTCGGTTCGTCGTCCCAATTTACCGACTCAGATGCTCAA
TTCAGTAACCCAGCTTATGATTGGGAAGTCTTCTCCAATTTCATTGTCTCTTATCCAATC
CACCCAATTCAATTGGTCTGAATCAAGAGATGAGAACGTTGAGACAATCCGTGGACTAAG
CTCGCCTCTGCTCTGTTGTGCCTCTTTGTCTCTAACTAGACCTAACGAGTCGACTCAGTC
AGTGGAAGGGAAGGATACAGTACAGCAGCAGAAGGGTCACTCGGTGAGTCAAAATGCGGA
GGAACGAGTTCTGATTAGTGAGGTTTTAGTAAGAACG
   
Sequence Name 10-K026766-022-430-F02C-KI01
GenBank Accession LN490498 [GenBank]
Sequencing primer gene specific primer, KI01: 5'-TCCTCACCATCTTTCGTTCTTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K026766-022-430-F02C-KI01
CCTCACTAATCAGAACTCGTTCCTCCGCATTTCGACTCACCGAGTGACCCTTCTGCTGCT
GTACTGTATCCTTCCCTTCCACTGACTGAGTCGACTCGTAAGGTCTAGTTAGAGACAAAG
AGGCACAACAGAGCAAAGGCAACTTAATCCACCGAATGGCCCCACCTTCCCCTCCCCTGG
AT

Last Updated on 06.12.2018 16:11