Sequences from confirmation amplicon


Confirmation sequences for line 430G06 and hit At1g07570 are shown.
Line ID 430G06
Locus At1g07570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2332753
Primers used for PCR MC73 - 8474, show primer details
   
Sequence Name 25-K035350-022-430-G06C-8409
GenBank Accession LN497348 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K035350-022-430-G06C-8409
GAGCCATTCTCAATTGAATATATGCCATCAATATACTCATTTCTTTTTCTTCATTGGTGA
AGGTGGATTCGGTTGTGTTTTCAAAGGATGGATTGATGAGAAGTCTCTCACTGCCTCAAG
ACCAGGCACGGGTTTGGTTATTGCCGTCAAAAAGCTTAACCAAGATGGTTGGCAAGGTCA
CCAGGAGTGGCTGGTAATAATGCTATAAGTTAAAATATGCCAATTTTGGTTCTTGTTTGT
TTTTCATTAATCTTCAGTTTTGATGATTTTGCAGGCTGAAGTGAATTACCTTGGTCAGTT
TTCTCACCGTCACCTTGTGAAGCTGATTGGTTATTGCCTAGAGGATGAGCACCGTCTTCT
TGTTTACGAGTTCATGCCTCGGGGTAGCTTGGAGAATCATCTTTTCAGGAGTATGTCGTC
CTTACTCTTAAGTCTAATCCCAGGCTGAGGTCAAACTTAAAGCTTTGTTATGACGTTTGT
GAATATTCAGGTTTGTTTTTCTTGGGTTACAGGAGGTTTGTA
   
Sequence Name 73-K035350-022-430-G06C-MC73
GenBank Accession LN512843 [GenBank]
Sequencing primer gene specific primer, MC73: 5'-AGTACAAACCTCCTGTAACCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K035350-022-430-G06C-MC73
ACGTCATAACAAAGCTTTAAGTTTGACCTCAGCCTGGGATTAGACTTAAGAGTAAGGACG
ACATACTCCTGAAAAGATGATTCTCCAAGCTACCCCGAGGCATGAACTCGTAAACAAGAA
GACGGTGCTCATCCTCTAGGCAATAACCAATCAGCTTCACAAGGTGACGGTGAGAAAACT
GACCAAGGTAATTCACTTCAGCCTGCAAAATCATCAAAACTGAAGATTAATGAAAAACAA
ACAAGAACCAAAATTGGCATATTTTAACTTATAGCATTATTACCAGCCACTCCTGGTGAC
CTTGCCAACCATCTTGGTTAAGCTTTTTGACGGCAATAACCAAACCCGTGCCTGGTCTTG
AGGCAGTGAGAGACTTCTCATCAATCCATCCTTTGAAAACACAACCGAATCCACCTTCAC
CAATGAAGAAAAAGAAATGAGTATATTGATGGCATATATTCAATTGATGAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCCAAAT
GG

Last Updated on 06.12.2018 16:11