Sequences from confirmation amplicon


Confirmation sequences for line 431G07 and hit At5g62470 are shown.
Line ID 431G07
Locus At5g62470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25080544
Primers used for PCR M96a - 8474, show primer details
   
Sequence Name 23-K024845-022-431-G07C-8409
GenBank Accession LN496403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K024845-022-431-G07C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATTACACTTATTAATAGTTATGTCA
CCGACTCTTTCGTCCACATATCTCTAATTACCATTTAATCCCTTTTTCCTTGAGTTAATT
GTTTTGTTAATATGGTGTTTTAGGTTTGAGAAGATGTAGCAAGAGCTGCAGATTGAGATG
GACTAATTATCTTCGACCCGGTATTAAGCGTGGAAATTTTACTGAGCATGAAGAGAAGAC
AATTGTTCATCTTCAAGCCCTTTTAGGCAACAGGTATATTTTAATGCACATGATCCATAT
TTTTCCAAAAAGTTACCTTTCTTGGGTATAAGATCTAAATATGTTTTTCTTTGTATATAG
ATGGGCAGCCATAGCATCATACCTTCCAGAAAGGACAGACAATGATATAAAGAACTATTG
GAACACTCACTTGAAGAAGAAGCTCAAAAAGATTAATGAATCTGGTGAAGAAGATAATGA
TGGTGTCTCTTCATCAAACACTAGTTCACAAAAGAACCATCAAAGCACTAACAAAGGTCA
ATGGGAAAGAAGACTTCAGACAGACATTAACATGGCAAAACAAGCTCTTT
   
Sequence Name 24-K024845-022-431-G07C-M96a
GenBank Accession LN496843 [GenBank]
Sequencing primer gene specific primer, M96a: 5'-GACAAGGCCTCACAAAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K024845-022-431-G07C-m96a
CATGTTAATGTCTGTCTGAAGTCTTCTTTCCCATTGACCTTTGTTAGTGCTTTGATGGTT
CTTTTGTGAACTAGTGTTTGATGAAGAGACACCATCATTATCTTCTTCACCAGATTCATT
AATCTTTTTGAGCTTCTTCTTCAAGTGAGTGTTCCAATAGTTCTTTATATCATTGTCTGT
CCTTTCTGGAAGGTATGATGCTATGGCTGCCCATCTATATACAAAGAAAAACATATTTAG
ATCTTATACCCAAGAAAGGTAACTTTTTGGAAAAATATGGATCATGTGCATTAAAATATA
CCTGTTGCCTAAAAGGGCTTGAAGATGAACAATTGTCTTCTCTTCATGCTCAGTAAAATT
TCCACGCTTAATACCGGGTCGAAGATAATTAGTCCATCTCAATCTGCAGCTCTTGCTACA
TCTTCTCAAACCTAAAACACCATATTAACAAAACAATTAACTCAAGGAAAAAGGGATTAA
ATGGTAATTAGAGATATGTGGACGAAAGAGTCGGTGACATAACTATTAATAAGTGTAATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11