Sequences from confirmation amplicon


Confirmation sequences for line 435D07 and hit At3g15850 are shown.
Line ID 435D07
Locus At3g15850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5360293
Primers used for PCR SR15 - 8474, show primer details
   
Sequence Name 40-K103676-0022-435-D07C-SR15
GenBank Accession LN503683 [GenBank]
Sequencing primer gene specific primer, SR15: 5'-CTAATAATGTTGGGGACTTGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K103676-0022-435-D07C-SR15
ACACCTACATTTTGCATCCGCTGGCTCTAGCGGTTGCTTTATACGCAATGGGTGGCTTTC
CATTCATCGTTTGGGGAATGGTATGTTCTGTGGCTAGTCTTGCATTTTGAATCTATTCAC
TGTTAATTTTTCATAACTAGCTGATAGGATTATGATGTGTTTTAGCTCGGCCAAAACAAG
ATAATTCTACCAGTTTTTTTTGTGTAGCTATCAATTTGCTGGGCCAAATCTCCTCACTTG
ATTTCCCTCTTGCGTTTTCTTGTAATTTAGTGCAACTTAGATTATTGACCTTAGGCACAC
AAGAAAAATGTAGAAAATCTACATGGATTAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 56-K103677-0022-435-D07C-8409
GenBank Accession LN509077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103677-0022-435-D07C-8409
TTTCTTGTGTGCCAAGGTCAATAATCTAAGTTGCACTAAATTACAAGAAAACGCAAGAGG
GAAATCAAGTGAGGAGATTTGGCCCAGCAAATTGATAGCTACACAAAAAAAACTGGTAGA
ATTATCTTGTTTTGGCCGAGCTAAAACACATCATAATCCTATCAGCTAGTTATGAAAAAT
TAACAGTGAATAGATTCAAAATGCAAGACTAGCCACAGAACATACCATTCCCCAAACGAT
GAATGGAAAGCCACCCATTGCGTATAAAGCAACCGCTAGAGCCAGCGGATGCAAAATGTA
GGTGGTTCGAAGGAATCGATAGAATGGCTGCTTCTCCAAGTCCCCAACATTATT

Last Updated on 06.12.2018 16:11