Sequences from confirmation amplicon


Confirmation sequences for line 437H12 and hit At3g55080 are shown.
Line ID 437H12
Locus At3g55080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20414990
Primers used for PCR PG35 - 8474, show primer details
   
Sequence Name 32-K101744-0022-437-H12C-8409
GenBank Accession LN500523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K101744-0022-437-H12C-8409
ATTTACAATTGAATATATTCTGATTCTTTTCCATCTTTATGCGATTGTTTAATGAGTGAT
TGTGAGAATAACCAATTTCATTTCCTACCAGAGGCTGTCTCCTGGTGTGATGCTTTATCT
TTAATGTCAAATACACTAACCATGTTAATAACTATATCAACTTCTGAGCAGGTCACTGTT
CGCCTCCAAAGTTATCTATGCTGGTGACTGCATGTTGAAAGTCCCTTTTAATGCGGTATA
TTGTTGTCTACTGAAACGAGCTTTTCTGTTATCATGTGTCTTAAGAAGTATTGGAAATTC
TTTTTATGCCATTAGTGTTGTTCTAACTTAAAATTTCAGCAAATAACTCCAGATGAGCTC
CCCTCGGATATTAGAGTTTTATTGAGCAATGAAGTTGGGAATATCGGAATGCTTGCTGCT
GTTCTAATAAGAGAGAAGAAAATGGGTCAAGTAAGCTAGGGCATCAACATTTGAGTAATT
GACTTAAAATAAGTCTCCCATTTANNGTGAGTG
   
Sequence Name 32-K101750-0022-437-H12C-PG35
GenBank Accession LN500524 [GenBank]
Sequencing primer gene specific primer, PG35: 5'-CACTCACATGAAATGGGAGACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K101750-0022-437-H12C-PG35
TGAGCCCTAGCTACTTGACCCATTTTCTTCTCTCTTATTAGAACAGCAGCAAGCATTCCG
ATATTCCCAACTTCATTGCTCAATAAAACTCTAATATCCGAGGGGAGCTCATCTGGAGTT
ATTTGCTGAAATTTTAAGTTAGAACAACACTAATGGCATAAAAAGAATTTCCAATACTTC
TTAAGACACATGATAACAGAAAAGCTCGTTTCAGTAGACAACAATATACCGCATTAAAAG
GGACTTTCAACATGCAGTCACCAGCATAGATAACTTTGGAGGCGAACAGTGACCTGCTCA
GAAGTTGATATAGTTATTAACATGGTTAGTGTATTTGACATTAAAGATAAAGCATCACAC
CAGGAGACAGCCTCTGGTAGGAAATGAAATTGGTTATTCTCACAATCACTCATTAAACAA
TCGCATAAAGATGGAAAAGAATCAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR PG36 - 8474, show primer details
   
Sequence Name 33-K101745-0022-437-H12C-8409
GenBank Accession LN500936 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101745-0022-437-H12C-8409
GAGCCTTTACATTGAATATATCATGAAGCCATTTACAATTAAACAAGACAAAATAACATG
ATTCAAAGTTGTACAATGTTTCAACTATTGATGACAAGACAGAACCATATTAAAGAACAT
CAAGACAAGTCATATAAAAATTAAGCTGCCACAGAAGGTATCAACAAAGAATCTGATCAT
TACATTGAAAGGGAACTATGAATAGAATTATTGAATAGAGAAAATGTGTCTAACAATCCA
TATTTTAATTGCCGGTAAACAGAAAACCGAGGTACTCGAAAAACGGATTTTGAAAAATAC
TTGCAAAGTTGCATCTTATTCAAAACATCATCAAGCCCACCTGCCATAGGTGGATTTCCC
AATCGAAAGAGTATTAGTTATCTTTGCTCCAGCAATTCGCTCTAACCAGGGTAAGAAGTT
ATTGTCCA
   
Sequence Name 33-K101751-0022-437-H12C-PG36
GenBank Accession LN500937 [GenBank]
Sequencing primer gene specific primer, PG36: 5'-TCTAGGCTTCGTTGGACAATAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101751-0022-437-H12C-PG36
TGGACAAGATACTAATACTCTTTCGATTGGGAAATCCACCTATGGCAGGTGGGCTTGATG
ATGTTTTGAATAAGATGCAACTTTGCAAGTATTTTTCAAAATCCGTTTTTCGAGTACCTC
GGTTTTCTGTTTACCGGCAATTAAAATATGGATTGTTAGACACATTTTCTCTATTCAATA
ATTCTATTCATAGTTCCCTTTCAATGTAATGATCAGATTCTTTGTTGATACCTTCTGTGG
CAGCTTAATTTTTATATGACTTGTCTTGATGTTCTTTAATATGGTTCTGTCTTGTCATCA
ATAGTTGAAACATTGTACAACTTTGAATCATGTTATTTTGTCTTGTTTAATTGTAAATGG
CTTCATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11