Sequences from confirmation amplicon


Confirmation sequences for line 438B10 and hit At1g20575 are shown.
Line ID 438B10
Locus At1g20575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7128724
Primers used for PCR NS38 - 8474, show primer details
   
Sequence Name 04-K102498-0022-438-B10C-8409
GenBank Accession LN487864 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102498-0022-438-B10C-8409
TTACAATTGAAATCAGCCGTCGTCTGTACAAAAATAAGGATTAAAATAATGTCAAATATA
GGGACTAAAAAGCAAGTTTCTCAAATTGGGCCTAATATAAATATGGGCCTATATAAAGCC
CAAAAATAAATCAGGAAAGCTTTAAAAGAGGTAAACTCGTCAATCGCATCTGAGTCGCTC
TTCTTTCCTTCCGCCATTTTTTTCTTTCTAGGTTGCAGAGTTAAAGGAGAAGGTTTTCAA
TTAGGGTTTTGTAGAGAGAAAGATGAGCCGAAGTTTGGGAATACCGGTGAAGCTTCTTCA
CGAGGCCTCAGGTCATATCGTGACGGTGGAGCTAAAGAGCGGCGAGCTTTACAGAGGAAG
TATGATTGAGTGTGAGGATAACTGGAACTGTCAGCTCGAGGATATTACTTATACCGCCAA
GGTAATCAACTGATACTCTGAAGATCTCGATTCATTTTATATGAGACTGTTTTTTGAACC
ATAGGTTTCGTTTCAATTTCATTGTT
   
Sequence Name 04-K102499-0022-438-B10C-NS38
GenBank Accession LN487865 [GenBank]
Sequencing primer gene specific primer, NS38: 5'-AAACAATGAAATTGAAACGAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102499-0022-438-B10C-NS38
ATCGAGATCTTCACAGTATCANTTGATTACCTTGGCGGTATAAGTAATATCCTCCAGCTG
ACAGTTCCAGTTATCCTCACACTCAATCATACTTCCTCTGTAAAGCTCGCCGCTCTTTAG
CTCCACCGTCACGATATGACCTGAGGCCTCGTGAAGAAGCTTCCCCGGTATTCCCAAACT
TCGGCTCATCTTTCTCTCTACAAAACCCTAATTGAAAACCTTCTCCTTTAACTCTGCAAC
CTAGAAAGAAAAAAATGGCGGAAGGAAAGAAGAGCGACTCAGATGCGATTGACGAGTTTA
CCTCTTTTAAAGCTTTCCTGATTTATTTTTGGGCTTTATATAGGCCCATATTTATATTAG
GCCCAATTTGAGAAACTTGCTTTTTAGTCCCTATATTTGACATTATTTTAATCCTTATTT
TTGTACAGACGACGGCTGATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATATCTAAATGTCTACATT

Last Updated on 06.12.2018 16:11