Sequences from confirmation amplicon


Confirmation sequences for line 441C09 and hit At1g49160 are shown.
Line ID 441C09
Locus At1g49160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18180992
Primers used for PCR YW02 - 8474, show primer details
   
Sequence Name 25-K024845-022-441-C09C-8409
GenBank Accession LN497279 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K024845-022-441-C09C-8409
TAGATTTCCCGGACATGATTACCGTTGATAAAAATGTTGTCACATTTGATATCTCGGTGT
ATTATCGGCGGATCTTGACTATGTAGATATTTCAATCCCGTTAAAATCTGTCTCGCCCAA
CACTTGACAGCCTTCATATTCACCTTTCTATGTTTCTTACGGTACCTTCAAGAAAACCAA
GATTTCGCTGCTTTAAACACAACTCCAAAACCGCTATTTTCGCGGCGACATAACAAGATA
GTATAAAAATGAGCAAAATACATACTGCCGGAGACTTCCTGAAGTGAACAACTCGGTAAT
TATGTTAACCGTCTTGTTCTTGTCATCGATCCACGAGTTATAAAACCTTATGATGTTTTT
ATGCTTCAGAGATTTCAAAAGCCGCACTTCGGAATATAGCCTCTCTAGACAATCTGGTGA
TTGCAAAAGATCGTCGATCCTTACTTGATTCCACGCTACTTCAATC
   
Sequence Name 26-K024845-022-441-C09C-YW02
GenBank Accession LN497755 [GenBank]
Sequencing primer gene specific primer, YW02: 5'-GAAGTAGATGGGATTGAAGTAGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K024845-022-441-C09C-YW02
AATCAAGTAAGGATCGACGATCTTTTGCAATCACCAGATTGTCTAGAGAGGCTATATTCC
GAAGTGCGGCTTTTGAAATCTCTGAAGCATAAAAACATCATAAGGTTTTATAACTCGTGG
ATCGATGACAAGAACAAGACGGTTAACATAATTACCGAGTTGTTCACTTCAGGAAGTCTC
CGGCAGTATGTATTTTGCTCATTTTTATACTATCTTGTTATGTCGCCGCGAAAATAGCGG
TTTTGGAGTTGTGTTTAAAGCAGCGAAATCTTGGTTTTCTTGAAGGTACCGTAAGAAACA
TAGAAAGGTGAATATGAAGGCTGTCAAGTGTTGGGCGAGACAGATTTTAACGGGATTGAA
ATATCTACATAGTCAAGATCCGCCGATAATACACCGAGATATCAAATGTGACAACATTTT
TATCAACGGTAATCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CAT

Last Updated on 06.12.2018 16:11