Sequences from confirmation amplicon


Confirmation sequences for line 444C11 and hit At5g35770 are shown.
Line ID 444C11
Locus At5g35770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13938404
Primers used for PCR BD56 - 8474, show primer details
   
Sequence Name 10-K028744-022-444-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K028744-022-444-C11C-8409
AACATATTTCATAAAATAGATTTTATAAA
   
Sequence Name 10-K028744-022-444-C11C-BD56
GenBank Accession LN490515 [GenBank]
Sequencing primer gene specific primer, BD56: 5'-CTAGGAAGATGGAAAGACAACGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K028744-022-444-C11C-BD56
ACTCATCGANACACACAACTTCATTTTTATAAAAATCTATTTCTATGACATATGTTCAAT
TGACGGGACTAATCAATCTCTGGAAGCTATAGATAGAGATATAACAACAGAAGCCCTAGT
GTAAAAGGAATAATGAGAGGAAGCCCTAGTCCCATGTCAAAAGAACAAAAAACAAATCAA
TGTGACAATAATACAAAACAGATGATATGTTAATTTATTAGGACAGATGAGAAGACTTTA
AATGAGTTAAGCTGAACATGAGATTCCGACTTTGCCAACAAGCGTGCCCTTCTCTCTTTC
ATTTCCAAGAAAGGATAGCTTTTAAGCCTTTGGTCTTGTTAAAAGTAGAGCTTCCCATAA
ATACACAAATATTACACTTATATATAGTAGATGTTTCTCTGTCTCTCTCATCCCTCTCCA
ACAATCCGTGGACCTACATTCTCTACATTTACGTTTATATTGTTATTTATTGTACATCTT
TATTCCTCATTAAACGCAAAGTAATGTATTTGACTTCGTACTCTAGCTCTATAGTACAAC
CTAAAATCAAATATTTCGAATTCTATACGAAAAGAAAAGATATCTTGTCAAACCAAAGGT
TTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAACAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT

Last Updated on 06.12.2018 16:11