Sequences from confirmation amplicon


Confirmation sequences for line 446A02 and hit At3g17730 are shown.
Line ID 446A02
Locus At3g17730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6064347
Primers used for PCR NU19 - 8474, show primer details
   
Sequence Name 10-K103925-0022-446-A02C-NU19
GenBank Accession LN490624 [GenBank]
Sequencing primer gene specific primer, NU19: 5'-CCATATTTGTCTGTATGATGGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K103925-0022-446-A02C-NU19
CTGATCACATGTACATGTAAAGGCAGATATATGTCTATAACTATATAAGTATATATTATG
CTAATAACGAGATCAAACGTGATCCTTTTTTTATAACGAAGGAATTGCAAGGAGCTTGCA
AGTAACGTTTGTCAAGAATCTCCAAAAAAGATAAAGCGAAAGATAAAAACAACAAAAAGA
AGAACGTCCAGAAAAAAGAAAGAAAGAGAGAGATATAATAATATAAGTAAAGAAAATTTT
AATAATTAATCAATCTAGTGACCATATAAGTTATTAATGTATGCGGTCAAGAAAGAAGAA
TATATATATATATATATATATATATATATAAGTTTGCGTGAGAGAAGATACATGATGTGT
ACCC
   
Sequence Name 26-K103926-0022-446-A02C-8409
GenBank Accession LN497892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K103926-0022-446-A02C-8409
CCATTTACATTGACTTGGGTACGTACTTATGACTTCACCTCATTTATCTTCTCTTACTCA
AACTTATATATATATATATATATATATATATATATTCTTCTTTCTTGAGCGCATACATTA
ATAACTTATATGGTCACTAGATTGATTAATTATTAAAATTTTCTTTACTTATATTATTAT
ATCTCTCTCTTTCTTTCTTTTTTCGGGACGTTCTTCTTTTTGTTGTTTTTATCTTTCGCT
TTATCTTTTTTGGAGATTCTTGACAAACGTTACTTGCGAGCTCCTTGCAATTCCTTCGAT
ATAAAAAAAGGATCACGTGTGATCTCGTTATTAACACAGTATATACGTATATAGTTAGAG
ACATATATGTGCCTTTACATGGACGTGTGTTGAGATGAAGCATATCGC

Last Updated on 06.12.2018 16:11