Sequences from confirmation amplicon


Confirmation sequences for line 453H07 and hit At2g20110 are shown.
Line ID 453H07
Locus At2g20110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8685861
Primers used for PCR 57kh - 8474, show primer details
   
Sequence Name 77-K113549-0022-453-H07C-57kh
GenBank Accession LN513579 [GenBank]
Sequencing primer gene specific primer, 57kh: 5'-TCTTTCTGATTTGCTAACCGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K113549-0022-453-H07C-57kh
CAACAGAAAGAACATAGTGCTTTCACATCCATTGGTTTAATTATGTTTGCCAAAAGAGAC
CTTCAAAAGAAAACATATTTTATAACTCAGTTTCTGCAAGTCTCAAAGAAAAGATTTAGC
TTTGTTAACACATCAGAAATTTACTTATATACAACTTTGGATGGGCCTAACGACGCATTG
CCTCCAGCACGAGAACCAGTTTGCGAAGATGTGGTTCTTCCGTTATTTGCCTGATACCAA
TAATTATACTATAAGATCTCTCATTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
   
Sequence Name 12-K113548w-0022-453-H07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113548w-0022-453-H07C-8409
TTTCAATGAGAGATCTTATAGTATAATTATTGGTATCAGGCAAATAACGGAAGAACCACA
TCTTCGCAAACTGGTTCTCGTGCTGGAGGCAATGCGTCGTTAGGCCCATCCAAAGTTGTA
TATAAGTAAATTTCTGATGTGTTAACAAAGCTAAATCTTTTCTTTGAGACTTGCAGAAAC
TGAGTTATAAAATATGTTTTCTTTTGAAGGTCTCTTTTGGCAAACATAATTAAACCAATG
GATGTGAAAGCACTATGTTCTGTTCTGGTTGCTGTAGCTGGGGAAGCTGCAAAGACATTA
ACAGAAAAACGGTTAGCAAATCAGAAAG

Last Updated on 06.12.2018 16:11