Sequences from confirmation amplicon


Confirmation sequences for line 454C04 and hit At4g34850 are shown.
Line ID 454C04
Locus At4g34850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16609422
Primers used for PCR JL06 - 8474, show primer details
   
Sequence Name 55-K032418-022-454-C04C-8409
GenBank Accession LN508710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K032418-022-454-C04C-8409
TTACAATTGAATATATCCTCATGGCTCATGAGAACTATAACCAGATGTTCTGGGCGGTTC
ATCCAGGTGGACCAGCCATCTTGAACCGGATTGAAAAGCGGCTTAACCTGTCGCCTGAGA
AGCTGAGTCCAAGCAGAAGAGCTCTCATGGACTATGGCAATGCGAGCAGCAATTCAATTG
TTTATGTGCTGGAGTATATGTTGGAGGAGAGCAAGAAAGTGAGGAACATGAATGAAGAAG
AAAACGAGTGGGGTCTGATTCTTGCTTTTGGACCTGGTGTTACATTTGAAGGCATTATCG
CTCGAAACCTTGATGTCTGAACAATAAGTTTTTACCTTGCAGATCGAGAAAGAACCCCTT
ATGCTTTAATAAAGACATGAAGCTAAACATACGAAATATTTTTTACGATGCTTAAATCTT
TCATAGTCTAAAAATATTTCACAAAGCAAGCAACGTAAAATTTGCAATTGAAAAATGAAG
AAAAGAGTCGTTATCAGCATTAAAGCCAGGAATCACGAAGAAGGTTCTTCTTAGCTCATC
TCTTTCCAACGTTTTCAACAAGTCCAGGAGTTGGATCTTCTCATAACGGGTTTCATCTGT
TTGTCTTCCTCAAGACAAACCATA
   
Sequence Name 55-K032418-022-454-C04C-JL06
GenBank Accession LN508711 [GenBank]
Sequencing primer gene specific primer, JL06: 5'-TCACATGTTGGTATGGTTTGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K032418-022-454-C04C-JL06
AGAGATCAACTCCTGGACTTGTTGAAACGTTGGAAAGAGATGAGCTAAGAAGAACCTTCT
TCGTGATTCCTGGCTTTAATGCTGATAACGACTCTTTTCTTCATTTTTCAATTGCAAATT
TTACGTTGCTTGCTTTGTGAAATATTTTTAGACTATGAAAGATTTAAGCATCGTAAAAAA
TATTTCGTATGTTTAGCTTCATGTCTTTATTAAAGCATAAGGGGTTCTTTCTCGATCTGC
AAGGTAAAAACTTATTGTTCAGACATCAAGGTTTCGAGCGATAATGCCTTCAAATGTAAC
ACCAGGTCCAAAAGCAAGAATCAGACCCCACTCGTTTTCTTCTTCATTCATGTTCCTCAC
TTTCTTGCTCTCCTCCAACATATACTCCAGCACATAAACAATTGAATTGCTGCTCGCATT
GCCATAGTCCATGAGAGCTCTTCTGCTTGGACTCAGCTTCTCAGGCGACAGGTTAAGCCG
CTTTTCAATCCGGTTCAAGATGGCTGGTCCACCTGGATGAACCGCCCAGAACATCTGGTT
ATAGTTCTCATGAGCCATGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATNTCGACGTGTCTAC
Primers used for PCR FP75 - 3144, show primer details
   
Sequence Name 45-K033040-022-454-C04C-3144
GenBank Accession LN505432 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K033040-022-454-C04C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTGGAAGCCATTTTCAATTGAATATTTCTTGCAGAAA
TTCTCCACATTGTCTTCTATTATCTGTGGAAGCTCTCTCGATAGCTTGAAGTTTATCCCT
TGTTCTGTTAGCCTTCCATCTATCGTCTTCTCTGTTTCTGCGCCATGAAAATGAAAATCC
ATATTTATCCAAGAATAGTCTCAAGGTATAGACATAACTGGATGCATAAATCTTGTGGTC
TTTTACCAGGTAAAAAGTTCTGTATTGCAGTATGAAGCTCAAGGAGAGG
   
Sequence Name 13-K033040-022-454-C04C-FP75
GenBank Accession LN491931 [GenBank]
Sequencing primer gene specific primer, FP75: 5'-AGCCTCTCTTTGAGCTTCATACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K033040-022-454-C04C-FP75
GATTATGCATCCAGTTATGTCTATACCTTGAGACTATTCTTGGATAAATATGGATTTTCA
TTTTCATGGCGCAGAAACAGAGAAGACGATAGATGGAAGGCTAACAGAACAAGGGATAAA
CTTCAAGCTATCGAGAGAGCTTCCACAGATAATAGAAGACAATGTGGAGAATTTCTGCAA
GAAATATTCAATTGAAAATGGCTTCCACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGG
AACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG

Last Updated on 06.12.2018 16:11