Sequences from confirmation amplicon


Confirmation sequences for line 454C10 and hit At3g20810 are shown.
Line ID 454C10
Locus At3g20810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7276633
Primers used for PCR MG02 - 8474, show primer details
   
Sequence Name 43-K027355-022-454-C10C-8409
GenBank Accession LN504715 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K027355-022-454-C10C-8409
CTCTTTCTTTTTTCTCCTATTTACGTTTTAATCAATGCTGTTGCTGGTAACCGCACCGTT
CCCGTGGAGGTGACTGCCATCATTTCTTCACTTAGTTCTTAGTTAGCTTGTGAGTGTTGA
TTAGTAGTCATTAGACGATGGTTTTAGCTTTAGTTGGCTCCTACGCTGCTCTAAGTTGCC
ATGTCCATATTTTTTTAGGGTTCTTCTTAAGAATGTTGAATCTCATAGAGTCGTGTCAAC
TGTATGATAAAAGGGTTTAGGGTCTAGGTTTGAAATAAGATTTGTATCTAGGTTAGATTA
TCACATAAAATCAGAATAAGAACATGACAATTTCACGAGCGTAAGATTTCTCATTTTTGC
TTCATAGAGATATGAGTCTTATTACCACTATTTAGTCTGAGCTAATAAGAAGATGGGTTG
GATCAGAATAATTTTTTCCGTAGCTGAAAGCTGGTG
   
Sequence Name 44-K027355-022-454-C10C-MG02
GenBank Accession LN505058 [GenBank]
Sequencing primer gene specific primer, MG02: 5'-GCATTTTAAACTTCACCAGCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K027355-022-454-C10C-MG02
ATCTGATCACCCATCTTCTTATTAGCTCAGACTAAATAGTGGTAATAAGACTCATATCTC
TATGAAGCAAAAATGAGAAATCTTACGCTCGTGAAATTGTCATGTTCTTATTCTGATTTT
ATGTGATAATCTAACCTAGATACAAATCTTATTTCAAACCTAGACCCTAAACCCTTTTAT
CATACAGTTGACACGACTCTATGAGATTCAACATTCTTAAGAAGAACCCTAAAAAAATAT
GGACATGGCAACTTAGAGCAGCGTAGGAGCCAACTAAAGCTAAAACCATCGTCTAATGAC
TACTAATCAACACTCACAAGCTAACTAAGAACTAAGTGAAGAAATGATGGCAGTCACCTC
CACGGGAACGGTGCGGTTACCAGCAACAGCATTGATTAAAACGTAAATATGGAGAAAAAA
GAAAGAGTAATTACCAATTTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11