Sequences from confirmation amplicon


Confirmation sequences for line 455A02 and hit At2g26330 are shown.
Line ID 455A02
Locus At2g26330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11209851
Primers used for PCR EQ74 - 8474, show primer details
   
Sequence Name 11-K103237w-0022-455-A02C-EQ74
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, EQ74: 5'-CTTACCATGAAGAAGATCCCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103237w-0022-455-A02C-EQ74
TCATAGAACAGAAGACTCCCCAAGTGAGAGAGGGAATAAGCTTGTAGGCTCACAAGATTT
CTGTGCTTGATGCTACTTAGCATCTCGAGTTCTGTTTCAAACTGTTTCATTGACTGTGGG
TTGTGAGAGTAAAGCCGCTTAATCGCAACCGGTTTACAATTCTTCAAAACACATTTGTAT
ACAGTGCTTGATGCTCCGTGCCCAATGATATACTTCTCACTTAGATTCTCTGTCATTCTC
ATGATATCCTCGTAAACGTGGAGTGCCATGTTCATATGAAGGATGACGAGCTTCGGTGTC
GAATAAGTTACTGATGTCACCAGGAAATTTAAGGATTAACTATTGGATTACATTGTAGGT
GAAGAACATTCGTAAAGTGGTTTGGAGAGTAGACCTGGTTTGTCAAGTGATCCATCAAGA
AAAGGAGGAGGATTATGCGGTCGGCAAGCTGCTATTAAGACCATGAGAAGGATCACAAGT
CCCCCAATAGCTATTCCAAGAATAGCTGCTCTAGAGATTGACACTGCAGACAAAACATGT
TTATGGCTAANATGATCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATTTCCG
   
Sequence Name 12-K103237w-0022-455-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103237w-0022-455-A02C-8409
AAGCCATTTACATGTAGATCATATTAGCCATAAACATGTTTTGTCTGCAGTGTCAATCTC
TAGAGCAGCTATTCTTGGAATAGCTATTGGGGGACTTGTGATCCTTCTCATGGTCTTAAT
AGCAGCTTGCCGACCGCATAATCCTCCTCCTTTTCTTGATGGATCACTTGACAAACCAGG
TCTACTCTCCAAACCACTTTACGAATGTTCTTCACCTACAATGTAATCCAATAGTTAATC
CTTAAATTTCCTGGTGACATCAGTAACTTATTCGACACCGAAGCTCGTCATCCTTCATAT
GAACATGGCACTCCACGTTTACGAGGATATCATGAGAATGACAGAGAATCTAAGTGAGAA
GTATATCATTGGGCACGGAGCATCAAGCACTGTATACAAATGTGTTTTGAAGAATTGTAA
ACCGGTTGCGATTAAGCGGCTTTACTCTCACAACCCACAGTCAATGAAACAGTTTGAAAC
AGAACTCGAGATGCTAAGTAGCATCAAGCACAGAAATCTTGTGAGCCTACAAGCTTATTC
CCTCTCTCANTTGGGGAGTCTTCTGTTCTATGACTATNTGGAAAATGGTAGCACTCTGGG
ATCTTCTCCATGGTAAG
Primers used for PCR KU46 - 8474, show primer details
   
Sequence Name 57-K118780-0022-455-A02C-KU46
GenBank Accession LN509554 [GenBank]
Sequencing primer gene specific primer, KU46: 5'-TATCCCTTCCAAGCTGTTAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118780-0022-455-A02C-KU46
CTCTGTATACCATAATGAGGTCACTATAGTGTGATCCAGGAACATGTATTGGGTTGGTGA
TCTATCTAATGTTGTGTTTCTTAAAATTGCTTGCAGGAGACTGGAAAATAATAACCTGAC
TGGTAATGTTGGTTCATTAGCCAACTGTCTCAGTCTCACTGTATTGTAAGTAGGCACCTT
TGGTTCTGAAACATTTTTTGTCCCTCTTTGTGCATCTTTTGCTAAGAATATAACCCTGCA
ATCTTCACTAACTCTTATAGGAATGTATCTCATAACAACCTCGTAGGTGATATCCCTAAG
AACAATAACTTCTCAAGATTTTCACCAGACAGGTATGGTAATTTAGCAGGTTTTGGTATT
GTGCATTTTGTTTTGTTTGCTAATATCTATGTTTATGTTTTTGGATAAAGCTTCATTGGC
AATCCTGGTCTTTGCGGTAGTTGGCTAAACTCACCGTGTCATGATTCTCGTCGAACTGTA
CGAGGTGATTACATTCTTCTAAAAGCTTCCATTCACAAAACCTAAGATAATTAAAGCTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 17-K118779-0022-455-A02C-8409
GenBank Accession LN494074 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118779-0022-455-A02C-8409
TTTATTATCTTAGGTTTTGTGAATGGAAGCTTTTAGAAGAATGTAATCACCTCGTACAGT
TCGACGAGAATCATGACACGGTGAGTTTAGCCAACTACCGCAAAGACCAGGATTGCCAAT
GAAGCTTTATCCAAAAACATAAACATAGATATTAGCAAACAAAACAAAATGCACAATACC
AAAACCTGCTAAATTACCATACCTGTCTGGTGAAAATCTTGAGAAGTTATTGTTCTTAGG
GATATCACCTACGAGGTTGTTATGAGATACATTCCTATAAGAGTTAGTGAAGATTGCAGG
GTTATATTCTTAGCAAAAGATGCACAAAGAGGGACAAAAAATGTTTCAGAACCAAAGGTG
CCTACTTACAATACAGTGAGACTGAGACAGTTGGCTAATGAACCAACATTACCAGTCAGG
TTATTATTTTCCAGTCTCCTGCAAGCAATTTTAAGAAACACAACATTAGATAGATCACCA
ACCCAATACATGTTCCTGGATCACACTATAGTGACCTCATTATTGTGTATACAGAGTTTT
AGTTTCATTCTACAAAAACAATTTCTTAACAGCTGG

Last Updated on 06.12.2018 16:11