Sequences from confirmation amplicon


Confirmation sequences for line 460A08 and hit At3g61560 are shown.
Line ID 460A08
Locus At3g61560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22777826
Primers used for PCR PE97 - 8474, show primer details
   
Sequence Name 30-K022562-022-460-A08C-8409
GenBank Accession LN499550 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K022562-022-460-A08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAATACGTTACCCG
ACGAGCCTTTAGATTCGTGACCCGAACCACAGAAATACGTTAACGACGTTAGTCTTTTTT
AAAGAAGGAATAAACGGACGTCAACGTTTAGTGCCTGGAACGTGGAGGCAACCAAGGATC
CAACAAAGGCCTAGTGGGCCTGGTGCGCTCTGTAACTCCTCTTTTTTACTTACATAAAGT
AACCGACAAGCGTAAGGAGATTCCGTATACGTGGCAGACTGGCAGTCATCGGTTCATATA
AGAATAATATCAACGGCTCACTCGTTTAAAAAAAAACAAACGGTCGATAGAACGACGTCG
TGGGACATTTCGTCAGTGAGTGGCAAAGTCTTTATCTCGGTTAGAGACTTTCTTTCTTTC
TCTTTTTTACATTCTTTGACATTTCAAACACATTAAAATTGCTTAGAAAAACATATGTTT
TTTTAGTCAACGTTACCGATATTTAGGAACATTTTTGTTTAAATCTCAAATTCCAACTAA
TTTGTTCCCATCTT
   
Sequence Name 31-K022562-022-460-A08C-PE97
GenBank Accession LN499983 [GenBank]
Sequencing primer gene specific primer, PE97: 5'-TGAATGTCTCTAAAGATGGGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K022562-022-460-A08C-PE97
ATTAGTTGGAATTTGAGATTTAAACAAAAATGTTCCTAAATATCGGTAACGTTGACTAAA
AAAACATATGTTTTTCTAAGCAATTTTAATGTGTTTGAAATGTCAAAGAATGTAAAAAAG
AGAAAGAAAGAAAGTCTCTAACCGAGATAAAGACTTTGCCACTCACTGACGAAATGTCCC
ACGACGTCGTTCTATCGACCGTTTGTTTTTTTTTAAACGAGTGAGCCGTTGATATTATTC
TTATATGAACCGATGACTGCCAGTCTGCCACGTATACGGAATCTCCTTACGCTTGTCGGT
TACTTTATGTAAGTAAAAAAGAGGAGTTACAGAGCGCACCAGGCCCACTAGGCCTTTGTT
GGATCCTTGGTTGCCTCCACGTTCCAGGCACTAAACGTTGACGTCCGTTTATTCCTTCTT
TAAAAAAGACTAACGTCGTTAACGTATTTCTGTGGTTCGGGTCACGAATCTAAAGGCTCG
TCGGGTAACGTATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATCATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCT
Primers used for PCR FN18 - 8474, show primer details
   
Sequence Name 59-K032008-022-460-A08C-8409
GenBank Accession LN509899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K032008-022-460-A08C-8409
CGGACTGAGCCTTTACATTGAATTATCCTATTTCTCCGTTCGTTCTCTCCCTCTCTCTCG
CTTCACGTTGGATCGAAATCGGACAAAAGTAAACGACAATGGCGGAAGAATTGGAGAAAT
CTATGCACAAGGAAGAATCTCTGATGGAGAAGATCGCTGATAAGATCCACGATCACGACT
CTTCGTCGTCGTCTGATTCCGAGCACGAGAAACCAGAGTCTCCGTCAGCTTTGAAGGCCA
AGATATATCGTTTGTTTGGTAGAGAGAAGCCTGTTCATAAGGTTCTCGGTGGTGGCTTAC
GTACGTTTTCTTCT
   
Sequence Name 60-K032008-022-460-A08C-FN18
GenBank Accession LN510164 [GenBank]
Sequencing primer gene specific primer, FN18: 5'-GAGAAGAAAACGTACGTAAGCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K032008-022-460-A08C-FN18
CGGCTTCCTCTCCAACAACGATATATCTTGGCCTTCAAGCTGACGGAGACTCTGGTTTCT
CGTGCTCGGAATCAGACGACGACGAAGAGTCGTGATCGTGGATCTTATCAGCGATCTTCT
CCATCAGAGATTCTTCCTTGTGCATAGATTTCTCCAATTCTTCCGCCATTGTCGTTTACT
TTTGTCCGATTTCGATCCAACGTGAAGCGAGAGAGAGGGAGAGAACGAACGGAGAAATAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11