Sequences from confirmation amplicon


Confirmation sequences for line 463C05 and hit At3g14030 are shown.
Line ID 463C05
Locus At3g14030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4646057
Primers used for PCR HF63 - 8474, show primer details
   
Sequence Name 95-K101272-0022-463-C05C-8409
GenBank Accession LN515342 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K101272-0022-463-C05C-8409
CGGAATGAGCCTTTACATTGAATATATACTCATACGCAGCATTTACAATATATTGAACGA
TCACATAAACAAGAAAATCATGAGGCAAAAATAAGAATTGGGAAAAGTCAGAAAGAAAAT
GAGGGAAAAGTTATTCAGAGAAAATATCTCACAGGAAATAAATGGGAAGATGTTGATTTC
AAGCTGCAGATACCAATAGCTGTGTAGTCTTTCCCTGTTGGTAGAATCTTCTATTGAATG
ATGGATCACATCACACACATTATGTATATCCNAGTGGGACACAATAAATGGAAGGAGTGT
ATCCAAATATGTCAGGTTTCCCGAGATTCTGTGCTTCTCGGATATTCACAGATTTGAAGT
ATCCATCTTGTCCAATGATGTGAGCTGTCTGGTAGCGGCAAGTCTCGGTCTTTTTACGTC
CGTCTAGAT
   
Sequence Name 95-K101278-0022-463-C05C-HF63
GenBank Accession LN515343 [GenBank]
Sequencing primer gene specific primer, HF63: 5'-CGATCTAGACGGACGTAAAAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K101278-0022-463-C05C-HF63
TTGGACAGATGGATACTTCAAATCTGTGAATATCCGAGAAGCACAGAATCTCGGGAAACC
TGACATATTTGGATACACTCCTTCCATTTATTGTGTCCCACTTGGATATACATAATGTGT
GTGATGTGATCCATCATTCAATAGAAGATTCTACCAACAGGGAAAGACTACACAGCTATT
GGTATCTGCAGCTTGAAATCAACATCTTCCCATTTATTTCCTGTGAGATATTTTCTCTGA
ATAACTTTTCCCTCATTTTCTTTCTGACTTTTCCCAATTCTTATTTTTGCCTCATGATTT
TCTTGTTTATGTGATCGTTCAATATATTGTAAATGCTGCGTATGAGTATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATA

Last Updated on 06.12.2018 16:11