Sequences from confirmation amplicon


Confirmation sequences for line 463E06 and hit At5g45840 are shown.
Line ID 463E06
Locus At5g45840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18594841
Primers used for PCR EQ75 - 8474, show primer details
   
Sequence Name 02-K103223-0022-463-E06C-8409
GenBank Accession LN486989 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103223-0022-463-E06C-8409
TTCGGACTGAGCCTTTACATTGAATATTGTAGTTCCCATTATTATCCTCGTCCTCGCGTT
CCAATCAAGATGCTCCATTTCCTTATCTGAACAAGAAGATATAGATTGGTTGGTGATAAT
CGTAATAAGCCTAACAAGATCTTGATGTAGAGGCATACTTATGTATATTACTAACCATGC
AAATGTTCGAAAAGAGTTCCATTTGGAGCATATTCGAAAACCATCATCCTATTAAACGGT
TCATCTTCTTCGCAATAACCAATCAGATTAATAAAGTTCTTATGGTTGACTCTTGACATT
GTATCAATCTGCAAATATAAGCTCTCAAAACATGAGTCAAGTTTTGCGGTAAAAATGGTC
GAAGAAAAACTGTTTATTTTAATTACCCTTCTGCGGTAAGTCATTTCCATAGCTCTTGTC
CATTCCCTAGTTTCCAAAATGGCGGTTGAAGCAACCGCAATCTCAACGCCNCTGGACAAA
GTTCCTTTATAGACNGTGTAACCATCANATGCTTCAATGATATTACTGAAATCTTCACAG
GCTGTTTCTAGTTCAGACCGGTTTAGCTTAGGTACACCTGAAAACATTTCTCACATTAAT
GTTAAACCGAANAGCAGAGCANAAATCAGTTTGTTTTAACAAATATTTTTACCAGTAACA
AAAGCTTTCTGCAATTGTCCACTCANACCAGTCTTCCATGGACCTATACTCTTCACAGCT
CTTTTCCGGAAGAAGAATATNACCGCTACTATNATCAGCATCGCTACGAANGCAGCAACA
CCGATNACAAC
   
Sequence Name 50-K103230-0022-463-E06C-EQ75
GenBank Accession LN507131 [GenBank]
Sequencing primer gene specific primer, EQ75: 5'-GAAAAATCGAAAGGGTTTAAGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K103230-0022-463-E06C-EQ75
TGCTTTCNAGCGATGCTGATATAGTAGCGGTTATATTCTTCTTCCGGAAAAGAGCTGTGA
AGAGTATAGGTCCATGGAAGACTGGTTTGAGTGGACAATTGCAGAAAGCTTTTGTTACTG
GTAAAAATATTTGTTAAAACAAACTGATTTTTGCTCTGCTTTTCGGTTTAACATTAATGT
GAGAAATGTTTTCAGGTGTACCTAAGCTAAACCGGTCTGAACTAGAAACAGCCTGTGAAG
ATTTCAGTAATATCATTGAAGCATTTGATGGTTACACTGTCTATAAAGGAACTTTGTCCA
GTGGCGTTGAGATTGCGGTTGCTTCAACCGCCATTTTGGAAACTAGGGAATGGACAAGAG
CTATGGAAATGACTTACCGCAGAAGGGTAATTAAAATAAACAGTTTTTCTTCGACCATTT
TTACCGCAAAACTTGACTCANGTTTTGAGAGCTTATATTTGCAGATTGATACAATGTCAA
GAGTCAACCATAAGAACTTTATTAATCTGATTGGTTATTGCGAAGAAGATGAACCGTTTA
ATAGGATGATGGTTTTCGAATATGCTCCAAATGGAACTCTTTTCGAACATTTGCATGGTT
AGTAATATACATAAGTATGCCTCTACATCAAGATCTTGTTAGGCTTATTACGATTATCAC
CAACCAATCTATATCTTCTTGTTCAGATAAGGAAATGGAGCATCTTGATTGGAACGCGAG
GACGAGGATAATAATGGGAACTACATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
Primers used for PCR KU47 - 8474, show primer details
   
Sequence Name 86-K118629-0022-463-E06C-KU47
GenBank Accession LN514701 [GenBank]
Sequencing primer gene specific primer, KU47: 5'-GACATTAGTCTCTGGTTCAGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K118629-0022-463-E06C-KU47
CCACTCATCGGTTTTCTCGGTTACTCCCGGTTTGTCCGCTGAAAGGAACCTCTCCGACCT
GCAAAACGAAGCATTATTTTACCACTAGGTCTTGTATTAAGAATCAAGAACACCAGTTTC
TTGTTAAGCAAGAAAGAAATAACTACTAAAACAGTTTTCTTGTTAAGCAAGAAAGTAAGA
ACCTTTGCTGCGTAATCATCGGTTAAGTATATTGCTGATGAGACAAGTTTAGTGTGTGAG
ATTGGAGGATTGAGCTCGTGCTCGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 67-K118628-0022-463-E06C-8409
GenBank Accession LN511909 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118628-0022-463-E06C-8409
ATTTACAATTGAATATATCCTGCACGAGCACGAGCTCAATCCTCCAATCTCACACACTAA
ACTTGTCTCATCAGCAATATACTTAACCGATGATTACGCAGCAAAGGTTCTTACTTTCTT
GCTTAACAAGAAAACTGTTTTAGTAGTTATTTCTTTCTTGCTTAACAAGAAACTGGTGTT
CTTGATTCTTAATACAAGACCTAGTGGTAAAATAATGCTTCGTTTTGCAGGTCGGAGAGG
TTCCTTTCAGCGGACAAACCGGGAGTAAACCGAGAAAACCGATGAGTGGTGATTTAGACC
AATCTTTATTGCCATTACCTCCTGAACCAGAGACTAATGT

Last Updated on 06.12.2018 16:11