Sequences from confirmation amplicon


Confirmation sequences for line 466F09 and hit At2g45660 are shown.
Line ID 466F09
Locus At2g45660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18808441
Primers used for PCR KN22 - 8474, show primer details
   
Sequence Name 63-K103246-0022-466-F09C-KN22
GenBank Accession LN510965 [GenBank]
Sequencing primer gene specific primer, KN22: 5'-CTTATTTGACCAAACTTCGCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103246-0022-466-F09C-KN22
ATTACACAAAATTAATAGATGCAAATTAGAAGATATATAGAACAATACATAAATTAAAGA
AGGTTGATGATTGAAGATAACCAAGAAAAACTAGTAAGGACTCTCCTAACCTAATAACCC
CCAAAAAGAACCCCAACTTTCTTATCTTTACCGAATAAAAAAACGAATGTGCAACCCTAA
CTTGAGACCCATAAGAATATATTATACCTTTTCAGAGAGCTTCTCGTTTTCTGCAGCTAG
AGCTTTCTCCTGCAAAAGTTTTAGATTCCAATTTGTAAAAAGCAAGCACAAGAGGCTTAC
TTACTTGGAATATTACACAATTTGCATACATACTTATATCTGTTTATATACACTCATAAA
AACTACTTTACCTTTTGCTTGAGCTGCTCAATTTGTTCCTTAAACACTTGAGTCTAAAAG
AGATGAGAAATTGAGTTATAATTATGAGTGGTTTCAGAAGATGATTCGAACAATACTTAT
NATGTGGAGAAACTTAAGCTGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCANAAATGTAGATGTCCGCAC
   
Sequence Name 15-K103247-0022-466-F09C-8409
GenBank Accession LN492933 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103247-0022-466-F09C-8409
GAGCCATTTACAATTGAACAGCTAAGTTTCTCCACATTATAAGTATTGTTCGAATCATCT
TCTGAAACCACTCATAATTATAACTCAATTTCTCATCTCTTTTAGACTCAAGTGTTTAAG
GAACAAATTGAGCAGCTCAAGCAAAAGGTAAAGTAGTTTTTATGAGTGTATATAAACAGA
TATAAGTATGTATGCAAATTGTGTAATATTCCAAGTAAGTAAGCCTCTTGTGCTTGCTTT
TTACAAATTGGAATCTAAAACTTTTGCAGGAGAAAGCTCTAGCTGCAGAAAACGAGAAGC
TCTCTGAAAAGGTATAATATATTCTTATGGGTCTCAAGTTAGGGTTGCACATTCGTTTTT
TTATTCGGTAAAGATAAGAAAGTTGGGGTTCTTTTTGGGGGTTATTAGGTTAGGAGAGTC
CTTACTAGTTTTTCTTGGTTATCTTCAATCATCAACCTTCTTTAATTTATGTATTGTTCT
ATATATCTCCTAATTTGCATCTATNAATTTTGTGTAATAATTCTATTTGAATGCAGTGGG
GATCTCATGAAAGCGAAGTTTGTCAAAATAAGAAG

Last Updated on 06.12.2018 16:11