Sequences from confirmation amplicon


Confirmation sequences for line 467C08 and hit At5g20885 are shown.
Line ID 467C08
Locus At5g20885 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7084689
Primers used for PCR LD13 - 8474, show primer details
   
Sequence Name 22-K030600-022-467-C08C-8409
GenBank Accession LN495990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K030600-022-467-C08C-8409
AATTGCACATACTTGAGCAAAGTCATCACAATGAGCTTCTTCATCGAAGAATTCTGGTCT
CATANGTCACTCAGCTTCTCTACAAAATGGCTGTTCTCATCACCGTCTTGAGATGGATCC
TCGCTTGGATCTTACGCTACCGATCCAGATCCAGATCCACCTCTTCTTCCACTTCCGCCT
GTCCTTCGATTTCCTCACAGGCGATCAAAGAAAGCCTCTCCGTGACGACGTTCCGTGACG
CAGCTGAGAGATCTCCGGCGATGATCAACGACACCTGCGCTGTGTGTCTCGGAGATCTGG
AAGATGGAGATGAGGTTAGAGAACTCAGGAACTGTAGCCATATGTTTCACCGTGAGTGTA
TTGACCGGTGGTTGGATTATGAATGTTGCGGTGGTGATGAGAATAACGAAGGTGAGGAGG
ATAATCACCGTACGTGTCCTCTTTGTAGAACTCCGCTACTTGCTGCTAATACGACGTCGT
GTGGTGATTGGCCGACGAAGAACGAACCTAGCTGGGCCGTTGAACGGCTTCTCT
   
Sequence Name 61-K030600-022-467-C08C-LD13
GenBank Accession LN510426 [GenBank]
Sequencing primer gene specific primer, LD13: 5'-TTCTAATTGATCAAAATCGATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K030600-022-467-C08C-LD13
ATCGGAAAGACAAAAAAAAATGAAATCAGACGTGAAGATCATCTCCGAAGAGGTAGAGAA
GCCGTTCAACGGCCCAGCTAGGTTCGTTCTTCGTCGGCCAATCACCACACGACGTCGTAT
TAGCAGCAAGTAGCGGAGTTCTACAAAGAGGACACGTACGGTGATTATCCTCCTCACCTT
CGTTATTCTCATCACCACCGCAACATTCATAATCCAACCACCGGTCAATACACTCACGGT
GAAACATATGGCTACAGTTCCTGAGTTCTCTAACCTCATCTCCATCTTCCAGATCTCCGA
GACACACAGCGCAGGTGTCGTTGATCATCGCCGGAGATCTCTCAGCTGCGTCACGGAACG
TCGTCACGGAGAGGCTTTCTTTGATCGCCTGTG

Last Updated on 06.12.2018 16:11