Sequences from confirmation amplicon


Confirmation sequences for line 468B03 and hit At5g57530 are shown.
Line ID 468B03
Locus At5g57530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23301392
Primers used for PCR JB01 - 8474, show primer details
   
Sequence Name 09-K024601-022-468-B03C-8409
GenBank Accession LN490011 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K024601-022-468-B03C-8409
ATGAAGCCATTTACAATTGAATATATCGACAAGACATCCGGTTCAGGTTTTCAATCCAAG
AAAGAGTATTTGTTCGGAAAGATCGATATGAAGATAAAACTTGTTCCCGGAAATTCTGCC
GGAACCGTCACAGCTTACTACGTATGTACAACTTCTTATATGCTCATTCAATTAAATATT
GTGGCATTTATCTTGAATCTAACATTAAGAATGTTTTAACAGCTTTCTTCAAAAGGTGAG
ACATGGGATGAGATTGATTTCGAGTTCTTGGGTAACGTTACTGGACAGCCTTATGTTATC
CATACAAATGTCTTTACCGGAGGTAAAGGTAACCGCGAAATGCAGTTCTATCTCTGGTTT
GATCCCACTGCTGATTTCCACACTTACACCGTGCTTTGGAACCCTCTCAACATCATGTAA
GTAACCGATCAATCCTATTCAAGATAGACAAATATCGCTAGACGCTAATCGGGCGTTTAA
AGCCGCCTAGGGCTTGATCTTAATATGATCCACGACTGTTCTAAATATTATGTTTTGTTT
TTCTTGGAATCTCAAAGCTTT
   
Sequence Name 10-K024601-022-468-B03C-JB01
GenBank Accession LN490484 [GenBank]
Sequencing primer gene specific primer, JB01: 5'-GAAAGCTTTTGAGATTCCAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K024601-022-468-B03C-JB01
GAAATCAGCAGCGGGATCAAACCACATATAGAACTGCATTTCGCGGTTACCTTTACCTCC
GGGAAAGACATTTGTATGGATAACATAAGGCTGGCCAGCAACGTTACCCAAGTAACTCGC
AAATCAATCTCATCCCATGTCTCACCTTTTGAAGAAAGCTGTTAAAACATTCTTAATGTT
AGATTCCAGATAAATGCCACAATATTTAATTGAATGAGCATATAAGAAGTTGTACATACG
TAGTAAGCTGTGACGGTTCCGGCAGAATTTCCGGGAACAAGTTTTATCTTCATATCGATC
TTTCCGAACAAATACTCTTTCTTGGATTGAAAACCTGAACCGGATGTCTTGTCGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAACAAGAAAGAGTGATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTA

Last Updated on 06.12.2018 16:11