Sequences from confirmation amplicon


Confirmation sequences for line 471G11 and hit At2g34480 are shown.
Line ID 471G11
Locus At2g34480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14532822
Primers used for PCR PE98 - 8474, show primer details
   
Sequence Name 31-K022565-022-471-G11C-8409
GenBank Accession LN499984 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K022565-022-471-G11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAAAACAAAAATAACCGATAAAATCC
AAAACGGGAGAGAGCACAGCTTCAACATCGATTCCACCCCAAAAAAGAGTCAATCAGAGA
CGTTTTACATGAACAAGTTGGGCTTGTTGGCCTTGTAAGTCGTCTTCAGCTTCCTGCTTG
GTGGTCTAACCTTGCGGAACACCAATGGGAACTTGATCTTGCTGTTGTGGAACTGTTTAG
TGCTCTCTCTCTTGCACAGCTTGGCTGGGACAGTGGCTGTCTTGATGATCTGAATGCAAG
GGAATCTGACACGGTGACGGGATGCCATCTCAGTGTACATCTGCTCAACAGCTCCGTTAA
GAGTGGTGTCACGGTACTCCTTGTACATGTTGTGGTACCCAGTTCTACTCTGGTACCTCA
ACCAAATACCGAA
   
Sequence Name 32-K022565-022-471-G11C-PE98
GenBank Accession LN500411 [GenBank]
Sequencing primer gene specific primer, PE98: 5'-GATCAAGAACTTCGGTATTTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K022565-022-471-G11C-PE98
CAGAGTAGAACTGGGTACCACAACATGTACAAGGAGTACCGTGACACCACTCTTAACGGA
GCTGTTGAGCAGATGTACACTGAGATGGCATCCCGTCACCGTGTCAGATTCCCTTGCATT
CAGATCATCAAGACAGCCACTGTCCCAGCCAAGCTGTGCAAGAGAGAGAGCACTAAACAG
TTCCACAACAGCAAGATCAAGTTCCCATTGGTGTTCCGCAAGGTTAGACCACCAAGCAGG
AAGCTGAAGACGACTTACAAGGCCAACAAGCCCAACTTGTTCATGTAAAACGTCTCTGAT
TGACTCTTTTTTGGGGTGGAATCGATGTTGAAGCTGTGCTCTCTCCCGTTTTGGATTTTA
TCGGTTATTTTTGTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11