Sequences from confirmation amplicon


Confirmation sequences for line 473A01 and hit MUB3 are shown.
Line ID 473A01
Locus MUB3 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25831193
Primers used for PCR PF37 - 8474, show primer details
   
Sequence Name 50-K102872-0022-473-A01C-8409
GenBank Accession LN507127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K102872-0022-473-A01C-8409
CATTTACATGTAGTATCTCTATTTTTCTTCATTCGAATTTCAAAAGTAAGTAAGTCATCC
ACTTCAATTCATAACCGAAAAATAAAAGTAAATTGATAGTATGTAAAAGTAAAACATTCT
TTAAGAAAAATGAGATGTAAAATTTCACATACAAGATACAGTAAAGTGACCATGTGAATC
TTTTTTGTTCATAAAAAATCATTTTGGTTAATCTAGAGGAACATGGTGTGCGAATCTATG
ACCATAACTTCAAGGACACGATGATGAGTTGTGTTTTTCGTCTTTGCTTCTTGGAACTTT
GTGATTTTTTGATCTGTGAATCTTGTGATTAGTACGAATCATAAAGCCTTGTTTGGCTGT
TTCCCATTCACGAAAAAATAC
   
Sequence Name 50-K102873-0022-473-A01C-PF37
GenBank Accession LN507128 [GenBank]
Sequencing primer gene specific primer, PF37: 5'-AGTATTTTGTCGTGAATGGGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K102873-0022-473-A01C-PF37
TCACAAGATTCACAGATAAAAAATCACAAAGTTCCAAGAAGCAACGACAAAAGACACAAC
TCATCATCGTGTCCTTGAAGTTATGGTCATAGATTCGCACACCATGTTCCTCTAGATTAA
CCAAAATGATTTTTTATGAACAAAAAAGATTCACATGGTCACTTTACTGTATCTTGTATG
TGAAATTTTACATCTCATTTTTCTTAAAGAATGTTTTACTTTTACATACTATCAATTTAC
TTTTATTTTTCGGTTATGAATTGAAGTGGATGACTTACTTACTTTTGAAATTCGAATGAA
GAAAAATAGAGATACTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACNTTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11