Sequences from confirmation amplicon


Confirmation sequences for line 475C05 and hit At2g35940 are shown.
Line ID 475C05
Locus At2g35940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15089287
Primers used for PCR JU19 - 8474, show primer details
   
Sequence Name 25-K021056-022-475-C05C-8409
GenBank Accession LN497250 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K021056-022-475-C05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATCACCATTGATGGTGGCGG
GTCCATATTCCTCTGTTTCTCCTATTTTCACTCTTCTCCCTATAGGAATCCCATGGTGGG
TCTGCATGAAACCCTGATGGTGCGTGGACGACAAGCTATCACAATGAGGTAAACCTAACG
TGAGGGACACGCCATTGTTGTTTCCTGAGTACCTTTGCGCCATGAGCTCCTGGTCTGAGA
CTACATCAAATCTTGACATCTCATCCATTTGGTATTGCCCGAAATTACCCATGAAAGGGT
AGCCACCATCTGATCTTATCCCTTGCCGTTCTTCTAGAATCTTCATCGTGAGCTTCTCGT
TGGAGCTGAAATCCGCATTTATTGGCTGCATCATTGTCTCGTCGCTGGTTCTTAGTCTCT
TGGGGCTTCCTTGCATTCCAGTGACGCCTTCTAGGTCACCGTTGTGATTGGGATTCATAT
GGTAATTAGTGTCCGCCATTGGGCTCTTTTCTTGGTTACTTGTTGACTTT
   
Sequence Name 26-K021056-022-475-C05C-JU19
GenBank Accession LN497726 [GenBank]
Sequencing primer gene specific primer, JU19: 5'-CTAAGCAAACAGGACTCACTCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K021056-022-475-C05C-JU19
CACCAGGGCCAGGTACGTTTCAAAATGATTCTTTCTTGTGTTATTAGGTTACTTAAGAAA
GCGTTTTTAAGTAAATTTATTTTCTTTAATTATTTAGGTGTCGAACTGGTTTATAAACGC
GAGAGTTCGGTTATGGAAACCAATGGTGGAGGAGATGTACATGGAGGAAATGAAGGAGCA
GGCAAAGAACATGGGATCCATGGAAAAGACTCCTTTGGATCAAAGCAACGAAGATTCTGC
TTCAAAGTCAACAAGTAACCAAGAAAAGAGCCCAATGGCGGACACTAATTACCATATGAA
TCCCAATCACAACGGTGACCTAGAAGGCGTCACTGGAATGCAAGGAAGCCCCAAGAGACT
AAGAACCAGCGACGAGACAATGATGCAGCCAATAAATGCGGATTTCAGCTCCAACGAGAA
GCTCACGATGAAGATTCTAGAAGAACGGCAAGGGATAAGATCAGATGGTGGCTACCCTTT
CATGGGTAATTTCGGGCAATACCAAATGGATGAGATGTCAAGATTTGATGTAGTCT
Primers used for PCR JU20 - 8474, show primer details
   
Sequence Name 27-K021056-022-475-C05C-oriL
GenBank Accession LN498189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K021056-022-475-C05C-oriL
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATGGGATGAACATACAGAACCAG
AAGAGATATGTGGCTCAGTTATTGCCCGACTTCGTTGCATAAACCCATCTCTCTAGAAGG
AGAAACCGAAACAGGTTATTATATACGTTTCTAGTTTTTAATTAGTATATAGTTTCTCAT
ACCATTGAACCAAAACAAAGAACAAAATTTAATTTTAGTCTTTGGTTATATATGGCCGAC
GGTTGTATATCGATTTTGTAATTTTTTTTTTTCGTTTCTATTGGGGGATGGATAGGGTTA
TATATGTATATGGATTAGTTACGTGTAATAGCTTGCAT
   
Sequence Name 28-K021056-022-475-C05C-JU20
GenBank Accession LN498658 [GenBank]
Sequencing primer gene specific primer, JU20: 5'-ATACACCCAATGCAAGCTATTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K021056-022-475-C05C-JU20
TAACTAATCCATATACATATATAACCCTATCCATCCCCCAATAGAAACGAAAAAAAAAAA
TTACAAAATCGATATACAACCGTCGGCCATATATAACCAAAGACTAAAATTAAATTTTGT
TCTTTGTTTTGGTTCAATGGTATGAGAAACTATATACTAATTAAAAACTAGAAACGTATA
TAATAACCTGTTTCGGTTTCTCCTTCTAGAGAGATGGGTTTATGCAACGAAGTCGGGCAA
TAACTGAGCCACATATCTCTTCGGGTTCTGTATGTTCATCCCATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTCCCAATTTTTTTTCAATTCAAAAATGTAGATGTCCCCACCGTTTTTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11