Sequences from confirmation amplicon


Confirmation sequences for line 476A11 and hit At5g27420 are shown.
Line ID 476A11
Locus At5g27420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9685031
Primers used for PCR AS40 - 8474, show primer details
   
Sequence Name 25-K026692-022-476-A11C-8409
GenBank Accession LN497286 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K026692-022-476-A11C-8409
CCGGACATGAAGCCATTTACAAGTCCGAGCGGTGACTCGGTACGAGCAGACCGGTGGGCT
TTTCTTAGAAACCCATCGTTTCTCTGGAGGAATACGACGCCGGTTCCTTCGCCGAGAGTT
GAAGTCAATAATAAGGACGGTGAAGGAACATCGTCAGTTCAACATATCGGTACAGTTGGT
TCAACTAGCGGTTCCCTTAGGCTACCGGTTTAGTGTTCGGTGATGTTTTGTGCATTGTTC
ATAAACACGCAATAGATTTTACGTAATTCTTTAATTTTGTTTGTCTTTTTCTGTAAGATA
TTTAAATCGACAATCCAACGGAAAAAGGAGATTGTTTTACTTAATTTATTTTATACTCTT
CAAAAGTCAAACAAAGAACGAACTGGAGATTGGTCTTGTTCTTAAAAAAAAAAAAAGTCT
TGTTCGTCGACCAAAGAATACATGGAACTGGAAGATAGAGACTACAACTTTTTTTTATAG
CGGTAATGGAATGTATTATCTAGTTCTGAATTCATGTGTTTATTTCTCGATGAATACGAT
GAACGACTAAAAAAAGAGAGGAACTTTTTTACAAACAAAT
   
Sequence Name 29-K026692-022-476-A11C-AS40
GenBank Accession LN499139 [GenBank]
Sequencing primer gene specific primer, AS40: 5'-AGTACAAATTTTTGTTTTGGTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K026692-022-476-A11C-AS40
AGAAAAAAAAAACGTTGTATTTCCCCAAAAAAAAAACAATCATTAATTGGAAACAAATTT
GTTGGTTATATAGTTCCTCTCTTTTTTTAGTCGTTCATCGTATTCATCGAGAAATAAACA
CATGAATTCAGAACTAGATAATACATTCCATTACCGCTATAAAATAAGGTTGTAGTCTCT
ATCTTCCAGTTCCATGTATTCTTGGGCCGACAAACAAAACTTTTTTTTTTTTAAAAAAAA
AAACCAATCCCCAGTTCGTTCTTGGTTGGCCTTTGGAAGAGTATAAAATAATTTAGGTAA
AACATTCCCCTTTTCCCGTGGGATGGCCAATTTAAATTTTTTCCGGAAAAAGACAACCAA
ATTTAAGGATTTCCGTAAAACCTTTGGGGGGTTTTTGAACAAGGCAAAAAAATTCCCCAA
ACACT

Last Updated on 06.12.2018 16:11