Sequences from confirmation amplicon


Confirmation sequences for line 479D02 and hit At3g25410 are shown.
Line ID 479D02
Locus At3g25410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9215277
Primers used for PCR RK72 - 8474, show primer details
   
Sequence Name 52-K020358-022-479-D02C-8409
GenBank Accession LN507680 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K020358-022-479-D02C-8409
TAGATTTCCCGGACATGAAGCCATTTACATGATCCAATTAGAAGACCACTAAGCAAAGGT
GTAAAGATTACAGAAGCAATAGTTGTAGAACTAGTGAGAAGAATGCTCATGGCCACATCA
GCTTTACTCAGAGAACTCGCGTAACTAGACAACTGAGCCCCAGCCACACAACACGTGAGT
ATAAAACCAGCGTAGAAGGTCCTCGGCATCCCAAATGCATTAGCAACTAAAACCCCGAGA
AGCGGTTTCAAAACGTACTGAGCAACAAACCCAACAGATAACGGCACTGGTCTGCAACCA
AACAATGTTCTCACAAACGTTAGAAAACCAGCTTAACTAAACTGATCATAAAACTTATAA
GAGAATGCTCCAAGTACCTTTTGAATGCAAGAGCAAAATCGTCAACTGAAAGCTGA
   
Sequence Name 56-K020358-022-479-D02C-RK72
GenBank Accession LN508972 [GenBank]
Sequencing primer gene specific primer, RK72: 5'-AATTCAGCTTTCAGTTGACGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K020358-022-479-D02C-RK72
TGCTCTTGCATTCAAAAGGTACTTGGAGCATTCTCTTATAAGTTTTATGATCAGTTTAGT
TAAGCTGGTTTTCTAACGTTTGTGAGAACATTGTTTGGTTGCAGACCAGTGCCGTTATCT
GTTGGGTTTGTTGCTCAGTACGTTTTGAAACCGCTTCTCGGGGTTTTAGTTGCTAATGCA
TTTGGGATGCCGAGGACCTTCTACGCTGGTTTTATACTCACGTGTTGTGTGGCTGGGGCT
CAGTTGTCTAGTTACGCGAGTTCTCTGAGTAAAGCTGATGTGGCCATGAGCATTCTTCTC
ACTAGTTCTACAACTATTGCTTCTGTAATCTTTACACCTTTGCTTAGTGGTCTTCTAATT
GGATCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATT

Last Updated on 06.12.2018 16:11