Sequences from confirmation amplicon


Confirmation sequences for line 480C06 and hit At5g57180 are shown.
Line ID 480C06
Locus At5g57180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23169686
Primers used for PCR PB05 - 8474, show primer details
   
Sequence Name 08-K102498-0022-480-C06C-8409
GenBank Accession LN489681 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K102498-0022-480-C06C-8409
AATTACTAATTCAGCATTAATGAGAACACACTCCTCAACAGCCACGTGGCAGAATACTAA
TGGCCAAAACAGAATCCCGAATAATTAATTATTTCAAACTAACCATTAACTAAAGAAAAA
TAAACATAGAAAATATCGAATTTTAACGGCGAGGGATTTGAGATTAAGAAGAGATTAGTG
TTAAACATACATTGACATCGACAGCTTCCGATCCCTGAATCTCGTCGGGAAACGGCGACG
TTTTATCAGACCAAGCTTCCAAAACGCCGTCGTAATCAAGCTTCAACAACGGACCTGTTC
TCTTCAAACTCGTCTCCTCCGTATCTTCCAAGCTTTTCGATTCCGTAATCAATGTCGTCG
TCACCGTCATTTTCTTCTTCTTCTTCTTCTTATTACTCTTCTCTCCAGAGATTACCACCT
TCTTACCGTCGGATTTCTCCTCATCAACGGTGGAGATTGCAGTTTCGGTTTTGACAGTTT
GAATTCGCGGCGAGATCTGTTCGAAATCAACGGTATGAATCTTCCACAATTTTGTGTCGT
CGTTTTCTCTGAGAGCACTTCGTAATCCAAGCCCTAACGGGAAATTGAATCCGTTAGAGC
TTCGATTCCATGAATTGATCATCATCATCTGTTCTAGTTTACCAATCCGATTAGAAGAAC
TTCTTCCACCGCGATTGATCATTCCCGGAACCCTAGATTCATAAATCCCCGAATTTGAAT
TCGATTCCACCACAGTCCCCATTATACTATCGATTCCTTCTTCCATATCCTCATCGAGAA
TCGATTCAGCATCGAATTCGTCG
   
Sequence Name 08-K102499-0022-480-C06C-PB05
GenBank Accession LN489682 [GenBank]
Sequencing primer gene specific primer, PB05: 5'-ATTTTCTGTTTCATCCAACGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K102499-0022-480-C06C-PB05
CTCCGGTGGAGATTGCTACGGTGGTGAAATCGAAAAGTTTGATTTCTCCGACGAATTCGA
TGCTGAATCGATTCTCGATGAGGATATTGAAGAAGGAATCGATAGTATAATGGGGACTGT
GGTGGAATCGAATTCAAATTCGGGGATTTATGAATCTAGGGTTCCGGGAATGATCAATCG
CGGTGGAAGAAGTTCTTCTAATCGGATTGGTAAACTAGAACAGATGATGATGATCAATTC
ATGGAATCGAAGCTCTAACGGATTCAATTTCCCGTTAGGGCTTGGATTACGAAGTGCTCT
CAGAGAAAACGACGACACAAAATTGTGGAAGATTCATACCGTTGATTTCGAACAGATCTC
GCCGCGAATTCAAACTGTCAAAACCGAAACTGCAATCTCCACCGTTGATGAGGAGAAATC
CGACGGTAAGAAGGTGGTAATCTCTGGAGAGAAGAGTAATAAGAAGAAGAAGAAGAAGAA
AATGACGGTGACGACGACATTGATTACGGAATCGAAAAGCTTGGAAGATACGGAGGAGAC
GAGTTTGAAGAGAACAGGTCCGTTGTTGAAGCTTGATTACGACGGCGTTTTGGAAGCTTG
GTCTGATAAAACGTCGCCGTTTCCCGACGAGATTCAGGGATCGGAAGCTGTCGATGTCAA
TGTATGTTTAACACTAATCTCTTCTTAATCTCAAATCCCTCGCCGTTAAAATTCGATATT
TTCTATGTTTATTTTTCTTTAGTTAATGGTTAGTTTGAAATAATTAATTATTCGGGATTC
TGTTTTGGCCATTAGTATTCTGCCACGTGGCTGTTGAGGAGTGTGTTCTCATTAATGCTG
AATT

Last Updated on 06.12.2018 16:11