Sequences from confirmation amplicon


Confirmation sequences for line 481E12 and hit At1g59830 are shown.
Line ID 481E12
Locus At1g59830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22021128
Primers used for PCR NK22 - 8474, show primer details
   
Sequence Name 02-K102723-0022-481-E12C-8409
GenBank Accession LN486982 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K102723-0022-481-E12C-8409
ATTTACATTGAAATCTCCGGTCTGATCCCGACGATCGTTGTCGATGGGGAATATCTCCTC
GTGGTGCTGGTTACACGTTTGGACAGGACATTGCTACTCAGTTTAATCATAACAATGGAC
TGAGTCTGATCTCAAGAGCGCATCAACTTGTAATGGAAGGCTATAATTGGTGTCAGGTAT
ATAATATGTGCATAACAATAGATTGGCATATGTGTTGAATAGGGTTCTGGTTGCAAAAAT
GCTGAATCTTTCGGTGTGTTGATAGGAAAAGAACGTAGTGACAGTGTTTAGTGCACCGAA
CTACTGTTACAGATGTGGAAACATGGCCGCAATTCTTGAGATTGGAGAAAAGATGGAACA
GAAC
   
Sequence Name 18-K102728-0022-481-E12C-NK22
GenBank Accession LN494256 [GenBank]
Sequencing primer gene specific primer, NK22: 5'-AGTTCTGTTCCATCTTTTCTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K102728-0022-481-E12C-NK22
TCTGTACAGTAGTTCGGTGCACTAAACACTGTCACTACGTTCTTTTCCTATCAACACACC
GAAAGATTCAGCATTTTTGCAACCAGAACCCTATTCAACACATATGCCAATCTATTGTTA
TGCACATATTATATACCTGACACCAATTATAGCCTTCCATTACAAGTTGATGCGCTCTTG
AGATCAGACTCAGTCCATTGTTATGATTAAACTGAGTAGCAATGTCCTGTCCAAACGTGT
AACCAGCACCACGAGGAGATATTCCCCATCCACAACGATCGTCGGGATCAGACCGGAGAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCACTCCAAAATTGGG

Last Updated on 06.12.2018 16:11