Sequences from confirmation amplicon


Confirmation sequences for line 482D07 and hit At2g45910 are shown.
Line ID 482D07
Locus At2g45910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18895480
Primers used for PCR KU90 - 8474, show primer details
   
Sequence Name 02-K103453-0022-482-D07C-KU90
GenBank Accession LN486995 [GenBank]
Sequencing primer gene specific primer, KU90: 5'-CTTGTTTGTGGTCACTGATCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103453-0022-482-D07C-KU90
GCCAATTTCAGTTGGTGCTGTGAAGGAGGAAGAAGTCAGGGTTCTCAGGGAAAAGGAAAG
GGAGAAAGTACATATGATCCTAGACGACTACCTTCGTATATGCCAGCAGAGAGGGGTATG
CCTCCATCTATTTTCCTTTTCTTTAGTGTTGATGTTATGCCCTTAATAGGGAACTTGTTT
TACTTCATTGGCTAGCACTACGATTATAGTGTCCTTATGATAGATAGTGTATTTATGATT
GTAGGTCAGGGCAGAAAAGATGTTTATTGAAATGGAATCCATCGAGAATGGAATTGTGCA
GCTCATCTCGGAGCTGGGAATCAGGAAACTTGTCATGGGAGCAGCAGCAGATAGACACTA
TTCAAGGTATGCTTATGGCGAGTCACTAAGCATTTTTTGGGATCTCTACTTATTCATACT
GAATCAACTCCTGTAGATCTGTGTGCTTCTTACTCTTCAATATCCTTATGTTTTGCTTCC
TCTATCAATACTTTGGCTTTCAGGAGAATGACGGATCTGAAGTCCAGGAAAGCCATCTTC
GTACGCCGAGAAGCACCTACTCTTTGTCAAATATGGTTTACTTGCAAAGGATACTTGGAT
TTCAAAATGACTTGGATCTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 18-K103454-0022-482-D07C-8409
GenBank Accession LN494268 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K103454-0022-482-D07C-8409
GCCATTTACATTGAATAGATCCAAGTCATTTTGAAATCCAAGTATCCTCTGTAAGTAAAC
CATATTTGACAAAGAGTAGGTGCTTCTCGGCGTACGAAGATGGCTTTCCTGGACTTCAGA
TCCGTCATTCTCCTGAAAGCCAAAGTATTGATAGAGGAAGCAAAACATAAGGATATTGAA
GAGTAAGAAGCACACAGATCTACAGGAGTTGATTCAGTATGAATAAGTAGAGATCCCAAA
AAATGCTTAGTGACTCGCCATAAGCATACCTTGAATAGTGTCTATCTGCTGCTGCTCCCA
TGACAAGTTTCCTGATTCCCAGCTCCGAGATGAGCTGCACAATTCCATTCTCGATGGATT
CCATTTCAATAAACATCTTTTCTGCCCTGACCTACAATCATAAATACACTATCTATCATA
AGGACACTATAATCGTAGTGCTAGCCAATGAAGTAAAACAAGTTCCCTATTAAGGGCATA
ACATCAACACTAAAGAAAAGGAAAATAGATGGAGGCATACCCCTCTCTGCTGGCATATAC
GAAGGTAGTCGTCTAGGATCATATGTACTTTCTCCCTTTCCTTTTCCCTGAAAACCCTGA
CTTCTTCCTCCTTCACAGCACCAACTGGAAATTTGGCGCCCACTAGGATGAATCAATGTG
AAAAAACAAGATCAGTGACCACAAACAAGA

Last Updated on 06.12.2018 16:11