Sequences from confirmation amplicon


Confirmation sequences for line 482D07 and hit At3g27190 are shown.
Line ID 482D07
Locus At3g27190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10042239
Primers used for PCR 42xk - 8474, show primer details
   
Sequence Name 24-K119110-0022-482-D07C-42xk
GenBank Accession LN497211 [GenBank]
Sequencing primer gene specific primer, 42xk: 5'-CTCCTTCTCACTTCCGTTTGAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K119110-0022-482-D07C-42xk
GCCGCTCTCATCAGCCCTTCGTTATCGGTTATTTTTCGATTCTCGCTCTTTGATTCTTGC
TTTAGTCAACAATCGAACTTTCTCTTATTCCGATCATCGTGTATCATCTCTCAGTTTCTT
GAAATTGGTGCTTCTACTGCATTTTATAGGGTTTTATCTTAGGGTTTTAGCTGTACGAAT
TTTCCGGTAGCATTTGTGCTTCCAAGATTAGAGAAATCTGGATCAAAATCAGTTTCTAGG
GTTTAATCCTTTGATTTACTTGAACATGTTTTCCTTTGACTTGTGTAGGAGTTACTGGTG
GTACAGCTTCTGGTAAGACGACGGTATGTGACATGATAATCCAGCAACTTCATGATCATC
GCATTGTTTTAGTTAACCAGGTTAGATTCTGTACCCGTCCCTGTTCTCAGTATTTTTGTG
TGTGGTACTCTGATGCTGTTTTGTTGATTAGGATTCGTTTTACCGTGGTTTGACATCTGA
AGAACTTGAGCATGTACAAGAATACAATTTTGATCAGCATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 01-K119109-0022-482-D07C-8409
GenBank Accession LN486801 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119109-0022-482-D07C-8409
TCAAATTGTATTCTTGTACATGCTCAAGTTCTTCAGATGTCAAACCACGGNTAAAACGAA
TCCTAATCAACAAAACAGCATCAGAGTACCACACACAAAAATACTGAGAACAGGGACGGG
TACAGAATCTAACCTGGTTAACTAAAACAATGCGATGATCATGAAGTTGCTGGATTATCA
TGTCACATACCGTCGTCTTACCAGAAGCTGTACCACCAGTAACTCCTACACAAGTCAAAG
GAAAACATGTTCAAGTAAATCAAAGGATTAAACCCTAGAAACTGATTTTGATCCAGATTT
CTCTAATCTTGGAAGCACAAATGCTACCGGAAAATTCGTACAGCTAAAACCCTAAGATAA
AACCCTATAAAATGCAGTAGAAGCACCAATTTCAAGAAACTGAGAGATGATACACGATGA
TCGGAATAAGAGAAAGTTCGATTGTTGACTAAAGCAAGAATCAAAGAGCGAGAATCGAAA
AATAACCGATAACGAAGGGCTGATGAGGAGCGGCGGGATCGTCGGTAGCCGAAAAAGAAG
AATTACTCAAACGGA

Last Updated on 06.12.2018 16:11