Sequences from confirmation amplicon


Confirmation sequences for line 489A08 and hit At2g42440 are shown.
Line ID 489A08
Locus At2g42440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17669940
Primers used for PCR JV16 - 8474, show primer details
   
Sequence Name 62-K032928-022-489-A08C-8409
GenBank Accession LN510675 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K032928-022-489-A08C-8409
CCATTACAATGAGAAAGGTGTAGGCCACTAAGAAGACAAATTGTGATTAAATAATGTAAA
GAGTATATGAAACCCACCTTCATCTACATCTTTATAAAAATTCCCCCTAAGCTAATGCTC
CTCCATTCTTAAAGCTACTCAAGGTTAATCATCAAAGACCTAAATACTTAGTAACACATA
ATAGTCATCACCATGACTGGCTCTGGCTCTCCTTGTGGAGCTTGTAAGTTTTTAAGAAGG
AGATGTGTAAAAGGATGTGTGTTTGCCCCATACTTTTGCCATGAACAAGGAGCTTCCCAT
TTTGCAGCAATTCACCAAGTGTTTGGAGCCAGCAATGCTTCTAAATTGCTCTCTCATCTT
CCCATGGAGGATCGACGTGAAGCCGCTACTACAATCTATTATGAAGCTCAAGCTCGCCGT
CAAGACCCCATCTATGGCTGCGTTTCTCATATCTTTTCCCTCCAACAACAAGTAAATATA
TAAAACTTCCTCTATTTAACCCTAGTCTTGATTTTGGATATGACAAAATGTGAATATCAT
ATGATATATGCATAATTTGTAAATACGGATCACTAATTTTCTTGTGGTGTTATTCGTAGG
TTGTGAATTTACAAACACAGCTAGAGATTCTAAAGCAACAAGCAACACAAAGCATGATGG
CTATTGATTCTCCCTCCTAAAAAAAACCC
   
Sequence Name 62-K032928-022-489-A08C-JV16
GenBank Accession LN510676 [GenBank]
Sequencing primer gene specific primer, JV16: 5'-TAGGGTTTTCTATGGAGGGAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K032928-022-489-A08C-JV16
CTTAGAATCTCTAGCTGTGTTTGTAAATTCACAACCTACGAATAACACCACAAGAAAATT
AGTGATCCGTATTTACAAATTATGCATATATCATATGATATTCACATTTTGTCATATCCA
AAATCAAGACTAGGGTTAAATAGAGGAAGTTTTATATATTTACTTGTTGTTGGAGGGAAA
AGATATGAGAAACGCAGCCATAGATGGGGTCTTGACGGCGAGCTTGAGCTTCATAATAGA
TTGTAGTAGCGGCTTCACGTCGATCCTCCATGGGAAGATGAGAGAGCAATTTAGAAGCAT
TGCTGGCTCCAAACACTTGGTGAATTGCTGCAAAATGGGAAGCTCCTTGTTCATGGCAAA
AGTATGGGGCAAACACACATCCTTTTACACATCTCCTTCTTAAAAACTTACAAGCTCCAC
AAGGAGAGCCAGAGCCAGTCATGGTGATGACTATTATGTGTTACTAAGTATTTAGGTCTT
TGATGATTAACCTTGAGTAGCTTTAAGAATGGAGGAGCATTAGCTTAGGGGGAATTTTTA
TAAAGATGTAGATGAAGGTGGGTTTCATATACTCTTTACATTATTTAATCACAATTTGTC
TTCTTAGTGGCCTACACCTTTCTCATTGTAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11